CCCP

製品コードS6494 バッチS649404

印刷

化学情報

Synonyms

Carbonyl cyanide m-chlorophenyl hydrazone, Carbonyl cyanide 3-chlorophenylhydrazone

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₉H₅ClN₄

分子量

204.62

CAS No.

555-60-2

Solubility (25°C)*

体外

DMSO

41 mg/mL (200.37 mM)

Ethanol

14 mg/mL (68.41 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	2.000mg/ml (9.77mM)	Taking the 1 mL working solution as an example, add 50 μL of 40 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	2.000mg/ml (9.77mM)	Taking the 1 mL working solution as an example, add 50 μL of 40 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	CCCP (Carbonyl cyanide m-chlorophenyl hydrazone, Carbonyl cyanide 3-chlorophenylhydrazone), an oxidative phosphorylation inhibitor, is a protonophore mitochondrial uncoupler that increases membrane permeability to protons, leading to a disruption in the mitochondrial membrane potential.
in vitro	CCCP, a drug perturbing adenosine triphosphate synthesis by transporting protons across the mitochondrial inner membrane, reduces the spheroid-forming cell (SFC) frequency, indicating sensitivity of stem-like cells towards oxidative phosphorylation (OXPHOS) inhibition.^[1]
in vivo	Carbonyl Cyanide 3-Chlorophenylhydrazone (CCCP), an oxidative phosphorylation (OXPHOS) uncoupler, kills bacterial cells and prevents forming of biofilm.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	cells freshly isolated from patient material or PDXs (patient-derived xenografts)
	濃度	25 µM
	反応時間	4 h
	実験の流れ	5× 10⁵ tumor spheroid cells are seeded into two wells of 6-well ultra-low attachment plates. After seven days, 25 µM CCCP or DMSO are added and cells are incubated for 4 h at 37 ℃. Cells are dissociated, stained with 200 nM TOTO-3 in PBS, and prepared for cell sorting as described. Living cells are sorted into 96-well ultra-low attachment plates containing 100 µL of fresh culture medium supplemented with 100 µg/mL streptomycin and 100 U/mL penicillin per well. Limiting dilution and determination of SFC frequency are performed.
動物実験	動物モデル	Female Balb/c mice
	投薬量	3 mg/kg
	投与方法	i.v.

参考

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961698/
https://pubmed.ncbi.nlm.nih.gov/28303897/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

UbiREAD deciphers proteasomal degradation code of homotypic and branched K48 and K63 ubiquitin chains [ Mol Cell, 2025, 85(7):1467-1476.e6]	PubMed: 40132582
Reprogramming cysteine metabolism via METTL14-SLC7A11 axis promotes the progression of NAFLD and hepatocellular carcinoma [ Life Sci, 2025, 380:123965]	PubMed: 40939958
Artemisinin synergizes with CCCP in autophagic cell death induction via ER stress in uveal melanoma [ iScience, 2025, 28(8):112972]	PubMed: 40686605
Porcine epidemic diarrhea virus induces mitophagy to inhibit the apoptosis and activation of JAK/STAT1 pathway [ Vet Microbiol, 2025, 303:110427]	PubMed: 39961163
Epstein-Barr Virus-Driven B-Cell Transformation under Germinal Center Hypoxia Requires External Unsaturated Fatty Acids [ Res Sq, 2025, rs.3.rs-6506954]	PubMed: 40313738
Advanced RPL19-TRAPKI-seq method reveals mechanism of action of bioactive compounds [ Nat Prod Bioprospect, 2025, 15(1):16]	PubMed: 40042546
TBC1D23 mediates Golgi-specific LKB1 signaling [ Nat Commun, 2024, 15(1):1785]	PubMed: 38413626
Gonococcal OMV-delivered PorB induces epithelial cell mitophagy [ Nat Commun, 2024, 15(1):1669]	PubMed: 38396029
Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis [ Redox Biol, 2024, 69:103002]	PubMed: 38142583
Fut2 Deficiency Promotes Intestinal Stem Cell Aging by Damaging Mitochondrial Functions via Down-Regulating α1,2-Fucosylation of Asah2 and Npc1 [ Research (Wash D C), 2024, 7:0343]	PubMed: 38550777

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。