CCCP

製品コードS6494 バッチS649405

印刷

化学情報

Chemical Structure Synonyms Carbonyl cyanide m-chlorophenyl hydrazone, Carbonyl cyanide 3-chlorophenylhydrazone Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C9H5ClN4
分子量 204.62 CAS No. 555-60-2
Solubility (25°C)* 体外 DMSO 41 mg/mL (200.37 mM)
Ethanol 41 mg/mL (200.37 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 CCCP (Carbonyl cyanide m-chlorophenyl hydrazone, Carbonyl cyanide 3-chlorophenylhydrazone), an oxidative phosphorylation inhibitor, is a protonophore mitochondrial uncoupler that increases membrane permeability to protons, leading to a disruption in the mitochondrial membrane potential.
in vitro

CCCP, a drug perturbing adenosine triphosphate synthesis by transporting protons across the mitochondrial inner membrane, reduces the spheroid-forming cell (SFC) frequency, indicating sensitivity of stem-like cells towards oxidative phosphorylation (OXPHOS) inhibition.[1]
in vivo

Carbonyl Cyanide 3-Chlorophenylhydrazone (CCCP), an oxidative phosphorylation (OXPHOS) uncoupler, kills bacterial cells and prevents forming of biofilm.

プロトコル(参考用のみ)

細胞アッセイ 細胞株 cells freshly isolated from patient material or PDXs (patient-derived xenografts)
濃度 25 µM
反応時間 4 h
実験の流れ

5× 105 tumor spheroid cells are seeded into two wells of 6-well ultra-low attachment plates. After seven days, 25 µM CCCP or DMSO are added and cells are incubated for 4 h at 37 ℃. Cells are dissociated, stained with 200 nM TOTO-3 in PBS, and prepared for cell sorting as described. Living cells are sorted into 96-well ultra-low attachment plates containing 100 µL of fresh culture medium supplemented with 100 µg/mL streptomycin and 100 U/mL penicillin per well. Limiting dilution and determination of SFC frequency are performed.
動物実験 動物モデル Female Balb/c mice
投薬量 3 mg/kg
投与方法 i.v.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

TBC1D23 mediates Golgi-specific LKB1 signaling [ Nat Commun, 2024, 15(1):1785] PubMed: 38413626
Gonococcal OMV-delivered PorB induces epithelial cell mitophagy [ Nat Commun, 2024, 15(1):1669] PubMed: 38396029
Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis [ Redox Biol, 2024, 69:103002] PubMed: 38142583
Fut2 Deficiency Promotes Intestinal Stem Cell Aging by Damaging Mitochondrial Functions via Down-Regulating α1,2-Fucosylation of Asah2 and Npc1 [ Research (Wash D C), 2024, 7:0343] PubMed: 38550777
PDGF-BB accelerates TSCC via fibroblast lactates limiting miR-26a-5p and boosting mitophagy [ Cancer Cell Int, 2024, 24(1):5] PubMed: 38169376
TRIM28 promotes porcine epidemic diarrhea virus replication by mitophagy-mediated inhibition of the JAK-STAT1 pathway [ Int J Biol Macromol, 2023, 10.1016/j.ijbiomac.2023.127722] PubMed: 37907173
A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis [ Cell Res, 2022, 32(2):119-138] PubMed: 34561617
Novel roles of RTN4 and CLIMP-63 in regulating mitochondrial structure, bioenergetics and apoptosis [ Cell Death Dis, 2022, 13(5):436] PubMed: 35508606
Neospora caninum Evades Immunity via Inducing Host Cell Mitophagy to Inhibit Production of Proinflammatory Cytokines in a ROS-Dependent Manner [ Front Immunol, 2022, 13:827004] PubMed: 35355995
Characterization of immune features and immunotherapy response in subtypes of hepatocellular carcinoma based on mitophagy [ Front Immunol, 2022, 13:966167] PubMed: 36304466

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。