CCG-1423

製品コードS7719 バッチS771901

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C18H13ClF6N2O3
分子量 454.75 CAS No. 285986-88-1
Solubility (25°C)* 体外 DMSO 90 mg/mL (197.91 mM)
Ethanol 4 mg/mL warmed with 50ºC water bath (8.79 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 CCG-1423 is a specific RhoA pathway inhibitor, which inhibits SRF-mediated transcription.
in vitro CCG-1423 selectively inhibits SRF-mediated transcription activated by Rho pathway signaling, and specifically inhibits LPA-stimulated DNA synthesis. CCG-1423 also selectively inhibits the proliferation of RhoC-overexpressing melanoma cells (A375M2 and SK-Mel-147), and strongly suppresses the Rho-dependent invasion by PC-3 cells. [1] CCG-1423 in combination with LY294002 enhances differentiation of mouse embryonic stem cells into intermediate mesoderm through BMP7-positive cells. [2] In H9c2 cells, CCG-1423 inhibits MRTF nuclear localization, and completely blocks STARS proximal reporter activity. [3] CCG-1423, as a Rho/MRTF/SRF pathway inhibitor, also represses both matrix-stiffness and TGF-beta-induced fibrogenesis in human colonic myofibroblasts. [4]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Melanoma lines that overexpress RhoC (A375M2 and SK-Mel-147), low RhoC-expressing lines (A375 and SK-Mel-28), transformed (SW962, PC-3, and SKOV-3) and nontransformed (WI-38) cell lines.
濃度 300 nM
反応時間 72 hours
実験の流れ Cells in normal culture medium are plated (2,000 per well) in a 96-well plate coated with laminin. After attachment, the medium is replaced with serum-free medium (0% FBS) with 30 μmol/L LPA with or without 300 nM CCG-1423. Fresh LPA with or without CCG-1423 is added at day 5 to ensure that LPA and compound are present throughout the experiment. On day 8, WST-1 reagent is added to the wells for 1 h and absorbance at 450 nm is read using a Victor plate reader.

カスタマーフィードバック

Data from [Data independently produced by , , Cell Signal, 2017, 31:87-95]

Data from [Data independently produced by , , Cellular Signalling, 2017, 31:87-95.]

Data from [Data independently produced by , , Biomed Pharmacother, 2018, 104:718-728]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

3D printed pore morphology mediates bone marrow stem cell behaviors via RhoA/ROCK2 signaling pathway for accelerating bone regeneration [ Bioact Mater, 2023, 26:413-424] PubMed: 36969106
The collateral activity of RfxCas13d can induce lethality in a RfxCas13d knock-in mouse model [ Genome Biol, 2023, 24(1):20] PubMed: 36726140
Osteogenesis-Inducing Chemical Cues Enhance the Mechanosensitivity of Human Mesenchymal Stem Cells for Osteogenic Differentiation on a Microtopographically Patterned Surface [ Adv Sci (Weinh), 2022, e2200053] PubMed: 35373921
Increased Expression of LMCD1 in Scleroderma-Associated Interstitial Lung Disease is Critical for Profibrotic Characteristics of Lung Myofibroblasts [ Arthritis Rheumatol, 2022, 10.1002/art.42344] PubMed: 36103378
Exosomes derived from human umbilical cord mesenchymal stem cells reduce tendon injuries via the miR-27b-3p/ARHGAP5/RhoA signaling pathway [ Acta Biochim Biophys Sin (Shanghai), 2022, 54(2):232-242] PubMed: 35130628
Lovastatin Inhibits RhoA to Suppress Canonical Wnt/β-Catenin Signaling and Alternative Wnt-YAP/TAZ Signaling in Colon Cancer [ Cell Transplant, 2022, 31:9636897221075749] PubMed: 35168393
Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement [ Oncogene, 2021, 10.1038/s41388-021-02101-z] PubMed: 34750518
RhoA enhances osteosarcoma resistance to MPPa-PDT via the Hippo/YAP signaling pathway [ Cell Biosci, 2021, 11(1):179] PubMed: 34627383
The Jumonji Domain-Containing Histone Demethylase Homolog 1D/lysine Demethylase 7A (JHDM1D/KDM7A) Is an Epigenetic Activator of RHOJ Transcription in Breast Cancer Cells [ Front Cell Dev Biol, 2021, 9:664375] PubMed: 34249916
Myeloid MKL1 Disseminates Cues to Promote Cardiac Hypertrophy in Mice [ Front Cell Dev Biol, 2021, 9:583492] PubMed: 33898415

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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