Datasheet

生物学的記述

Specificity	CDKN2A/p14ARF Antibody [A5L13] detects endogenous levels of total CDKN2A/p14ARF protein.
Background	CDKN2A/p14ARF, encoded by the INK4a/ARF locus, functions as a pivotal tumor suppressor protein that diverges from its p16INK4a sibling through alternative reading frame usage, primarily stabilizing p53 to enforce cell cycle checkpoints and apoptosis. It features a compact structure with two α-helical domains that mediate high-affinity binding to MDM2, sequestering this E3 ligase in the nucleolus to prevent p53 ubiquitination and degradation, thereby amplifying p53-dependent transcription of p21 and BAX while licensing DNA damage-induced G1 arrest and caspase activation. This nucleolar detention of MDM2 integrates p14ARF into the ARF-MDM2-p53 signaling axis, where hyperproliferative signals like oncogenic MYC or RAS trigger ARF induction via E2F1 derepression, creating a surveillance mechanism that couples aberrant mitogenic drive to p53 restoration; concurrently, p14ARF binds E2F1 to directly blunt its transactivation of S-phase genes and interacts with TOP1 to enhance rRNA synthesis while promoting NPM1 polyubiquitination for ribosome biogenesis control. p14ARF inhibits cyclin B1-CDK1 complexes for G2/M blockade and represses BCL6 transcriptional activity, ensuring robust antiproliferative barriers in epithelial and hematopoietic contexts. This position p14ARF as a guardian of tissue homeostasis during development and regeneration, ideal for researchers probing oncogene-induced senescence in co-culture assays or dissecting nucleolar stress responses with isoform-specific knockdowns. Inactivation via homozygous deletion or methylation unleashes MDM2 hyperactivity in cancers like melanoma, pancreatic adenocarcinoma, and gliomas. Its inducible expression and short half-life offer experimental precision for synthetic lethality screens targeting MDM2, with therapeutic restoration via gene therapy showing promise in p53-wildtype malignancies.

Specificity

CDKN2A/p14ARF Antibody [A5L13] detects endogenous levels of total CDKN2A/p14ARF protein.

Background

CDKN2A/p14ARF, encoded by the INK4a/ARF locus, functions as a pivotal tumor suppressor protein that diverges from its p16INK4a sibling through alternative reading frame usage, primarily stabilizing p53 to enforce cell cycle checkpoints and apoptosis. It features a compact structure with two α-helical domains that mediate high-affinity binding to MDM2, sequestering this E3 ligase in the nucleolus to prevent p53 ubiquitination and degradation, thereby amplifying p53-dependent transcription of p21 and BAX while licensing DNA damage-induced G1 arrest and caspase activation. This nucleolar detention of MDM2 integrates p14ARF into the ARF-MDM2-p53 signaling axis, where hyperproliferative signals like oncogenic MYC or RAS trigger ARF induction via E2F1 derepression, creating a surveillance mechanism that couples aberrant mitogenic drive to p53 restoration; concurrently, p14ARF binds E2F1 to directly blunt its transactivation of S-phase genes and interacts with TOP1 to enhance rRNA synthesis while promoting NPM1 polyubiquitination for ribosome biogenesis control. p14ARF inhibits cyclin B1-CDK1 complexes for G2/M blockade and represses BCL6 transcriptional activity, ensuring robust antiproliferative barriers in epithelial and hematopoietic contexts. This position p14ARF as a guardian of tissue homeostasis during development and regeneration, ideal for researchers probing oncogene-induced senescence in co-culture assays or dissecting nucleolar stress responses with isoform-specific knockdowns. Inactivation via homozygous deletion or methylation unleashes MDM2 hyperactivity in cancers like melanoma, pancreatic adenocarcinoma, and gliomas. Its inducible expression and short half-life offer experimental precision for synthetic lethality screens targeting MDM2, with therapeutic restoration via gene therapy showing promise in p53-wildtype malignancies.

使用情報

Application

WB, IP, IF, FCM

Dilution

WB	IP	IF	FCM
1:1000	1:80	1:250	1:120

Reactivity

Human

Source

Rabbit Monoclonal Antibody

MW

14 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/11876522/
https://pubmed.ncbi.nlm.nih.gov/9724636/

Application Data

WB

Validated by Selleck

Lane 1: PC-3, Lane 2: Hela