CeMMEC1 HCl

製品コードS8328 バッチS832801

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₉H₁₇ClN₂O₄

分子量

372.8

CAS No.

2095432-53-2

Solubility (25°C)*

体外

DMSO

23 mg/mL (61.69 mM)

Ethanol

1 mg/mL (2.68 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	CeMMEC1は、TAF1の第二ブロモドメインを阻害する（IC50=0.9 μM）N-メチルイソキノリノン誘導体です。
in vitro	CeMMEC1 only bound BRD4 very weakly but it shows high affinity for the bromodomains of CREBBP, EP300, BRD9 and the second bromodomain of TAF1.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	REDS3 cells
	濃度	10 μM
	反応時間	24 h
	実験の流れ	REDS3 cells were treated with 10 μM of every compound, and live-cell imaging pictures were taken to assess their ability to induce RFP expression 24 h later. From this primary screening 1,286 small molecules were selected as hits and rescreened in the follow-up part, in which REDS3 and WT KBM7 cells were treated in three-point dose response in order to exclude autofluorescent or toxic compounds. 80 small molecules were selected as hits and used to treat WT KBM7 cells and REDS3 cells in eight-point dose (twofold dilution, starting from 100 μM) response and three-point time course (24 h, 48 h and 72 h) to carefully select the best true hits (time- and dose-dependent RFP expression/no autofluorescence). UPLC-MS analysis was done to confirm purity and the correct mass of the small molecules selected; finally, 22 small molecules fulfilled the criteria as screening hits.

参考

https://pubmed.ncbi.nlm.nih.gov/27159579/

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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