Chloroquine

製品コードS6999 バッチS699904

印刷

化学情報

Synonyms

NSC-187208, CHQ, CQ

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₁₈H₂₆ClN₃

分子量

319.87

CAS No.

54-05-7

Solubility (25°C)*

体外

DMSO

64 mg/mL (200.08 mM)

Ethanol

64 mg/mL (200.08 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution

5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O

3.25mg/ml

Taking the 1 mL working solution as an example, add 50 μL 65 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Chloroquine is an antimalarial drug and autophagy/lysosome inhibitor. Chloroquine also suppresses Toll-like receptor-9 (TLR9) protein expression. Chloroquine is highly effective agianst SARS-CoV-2 (COVID-19) infection with EC50 of 1.13 μM in Vero E6 cells. Chloroquine has anti-HIV-1 activity.
in vitro	Chloroquine is a widely used autophagy inhibitor.^[5]
in vivo	The inhibition of autophagy by chloroquine potentiates the therapeutic effect of PI3K/AKT inhibitors in vitro and in vivo TNBC models, synergizing with taxane-based chemotherapy.^[6]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HL‐60 cells
	濃度	10 µM
	反応時間	48 h
	実験の流れ	One day before treatment, AML cells were seeded with a density of 2 × 10⁵ cells/mL. Fresh medium containing different concentrations of Ara‐C was added into the culture medium. Cells were further cultured for 24 or 48 h based on experimental purposes. For inhibition of autophagy, HL‐60 cells were treated with 200 nM bafilomycin A1 or 10 µM chloroquine. Overexpression and knockdown were performed by transfection. (Data sourced from selleck products)
動物実験	動物モデル	Female NOD/SCID athymic mice injected with MDAMB231 cells
	投薬量	30 mg/kg
	投与方法	o.g. (Data sourced from selleck products)

参考

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Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Engineered a dual-targeting HA-TPP/A nanoparticle for combination therapy against KRAS-TP53 co-mutation in gastrointestinal cancers [ Bioact Mater, 2024, 32:277-291]	PubMed: 37876556
Dysregulated CREB3 cleavage at the nuclear membrane induces karyoptosis-mediated cell death [ Exp Mol Med, 2024, 56(3):686-699]	PubMed: 38480902
Fut2 Deficiency Promotes Intestinal Stem Cell Aging by Damaging Mitochondrial Functions via Down-Regulating α1,2-Fucosylation of Asah2 and Npc1 [ Research (Wash D C), 2024, 7:0343]	PubMed: 38550777
Sonic hedgehog restrains the ubiquitin-dependent degradation of SP1 to inhibit neuronal/glial senescence associated phenotypes in chemotherapy-induced peripheral neuropathy via the TRIM25-CXCL13 axis [ J Adv Res, 2024, S2090-1232(24)00106-1]	PubMed: 38479571
Inhibiting autophagy enhanced mitotic catastrophe-mediated anticancer immune responses by regulating the cGAS-STING pathway [ Cancer Lett, 2024, 586:216695]	PubMed: 38325769
Pinacidil ameliorates cardiac microvascular ischemia-reperfusion injury by inhibiting chaperone-mediated autophagy of calreticulin [ Basic Res Cardiol, 2024, 119(1):113-131]	PubMed: 38168863
PLEKHM2 deficiency induces impaired mitochondrial clearance and elevated ROS levels in human iPSC-derived cardiomyocytes [ Cell Death Discov, 2024, 10(1):142]	PubMed: 38490981
ULK2 Is a Key Pro-Autophagy Protein That Contributes to the High Chemoresistance and Disease Relapse in FLT3-Mutated Acute Myeloid Leukemia [ Int J Mol Sci, 2024, 25(1)646]	PubMed: 38203816
Migrasomal autophagosomes relieve endoplasmic reticulum stress in glioblastoma cells [ BMC Biol, 2024, 22(1):23]	PubMed: 38287397
Disruption of Autophagic Flux and Treatment with the PDPK1 Inhibitor GSK2334470 Synergistically Inhibit Renal Cell Carcinoma Pathogenesis [ J Cancer, 2024, 15(5):1429-1441]	PubMed: 38356720

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。