Chloroquine

製品コードS6999 バッチS699904

印刷

化学情報

Synonyms

NSC-187208, CHQ, CQ

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₁₈H₂₆ClN₃

分子量

319.87

CAS No.

54-05-7

Solubility (25°C)*

体外

DMSO

64 mg/mL (200.08 mM)

Ethanol

64 mg/mL (200.08 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	3.250mg/ml (10.16mM)	Taking the 1 mL working solution as an example, add 50 μL 65 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Chloroquineは抗マラリア薬であり、オートファジー/リソソーム阻害剤です。ChloroquineはToll様受容体-9 (TLR9)タンパク質の発現も抑制します。ChloroquineはVero E6細胞においてEC50が1.13 μMでSARS-CoV-2 (COVID-19)感染に非常に有効です。Chloroquineには抗HIV-1活性があります。
in vitro	Chloroquine is a widely used autophagy inhibitor.
in vivo	The inhibition of autophagy by chloroquine potentiates the therapeutic effect of PI3K/AKT inhibitors in vitro and in vivo TNBC models, synergizing with taxane-based chemotherapy.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HL‐60 cells
	濃度	10 µM
	反応時間	48 h
	実験の流れ	One day before treatment, AML cells were seeded with a density of 2 × 10⁵ cells/mL. Fresh medium containing different concentrations of Ara‐C was added into the culture medium. Cells were further cultured for 24 or 48 h based on experimental purposes. For inhibition of autophagy, HL‐60 cells were treated with 200 nM bafilomycin A1 or 10 µM chloroquine. Overexpression and knockdown were performed by transfection. (Data sourced from selleck products)
動物実験	動物モデル	Female NOD/SCID athymic mice injected with MDAMB231 cells
	投薬量	30 mg/kg
	投与方法	o.g. (Data sourced from selleck products)

参考

https://pubmed.ncbi.nlm.nih.gov/25385822/
https://pubmed.ncbi.nlm.nih.gov/24273604/
https://pubmed.ncbi.nlm.nih.gov/32145363/

https://pubmed.ncbi.nlm.nih.gov/11166661/
https://pubmed.ncbi.nlm.nih.gov/33638615/
https://pubmed.ncbi.nlm.nih.gov/35761360/

+ 展开

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Indole-3-Propionic Acid Improves Alveolar Development Impairment via Targeting VAMP8-mediated SNAREs Complex Formation in Bronchopulmonary Dysplasia [ Adv Sci (Weinh), 2026, 13(19):e02610]	PubMed: 41650278
RPS6KA3/RSK2-mediated phosphorylation of DRAM2 promotes lysosomal targeting and autophagic flux in melanoma [ Autophagy, 2026, 1-20.]	PubMed: 42059423
ADCY7 dictates N-cadherin stability to inhibit HCC metastasis [ JHEP Rep, 2026, 8(6):101789]	PubMed: 42096912
LncRNA SNHG1 Knockdown Ameliorates HIV-1 gp120V3 Loop-Induced Microglial Neuroinflammation by Regulating the Autophagy Process [ Inflammation, 2026, 49(1):26]	PubMed: 41489751
The E3 ubiquitin ligase NEDD4 protects against nonesterified fatty acid-induced hepatic inflammatory injury in ketotic cows [ J Dairy Sci, 2026, 109(5):5594-5610]	PubMed: 41724477
Macromolecule-Loaded Hybrid Extracellular Vesicles via Ionic Lipid-Based Post-Loading for Intracellular Delivery: Functional Evaluation for Neurodegenerative Therapy [ J Extracell Biol, 2026, 5(3):e70125]	PubMed: 42022851
VCP downstream metabolite glycerol-3-phosphate (G3P) inhibits CD8+T cells function in the HCC microenvironment [ Signal Transduct Target Ther, 2025, 10(1):26]	PubMed: 39848960
MAPK13 phosphorylates PHGDH and promotes its degradation via chaperone-mediated autophagy during liver injury [ Cell Discov, 2025, 11(1):15]	PubMed: 39962071
Cytosolic cytochrome c represses ferroptosis [ Cell Metab, 2025, S1550-4131(25)00149-4]	PubMed: 40233758
Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane [ Nat Commun, 2025, 16(1):6271]	PubMed: 40623997

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。