Chloroquine

製品コードS6999 バッチS699904

印刷

化学情報

Chemical Structure Synonyms NSC-187208, CHQ, CQ Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C18H26ClN3
分子量 319.87 CAS No. 54-05-7
Solubility (25°C)* 体外 DMSO 64 mg/mL (200.08 mM)
Ethanol 64 mg/mL (200.08 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 3.250mg/ml (10.16mM) Taking the 1 mL working solution as an example, add 50 μL 65 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Chloroquine is an antimalarial drug and autophagy/lysosome inhibitor. Chloroquine also suppresses Toll-like receptor-9 (TLR9) protein expression. Chloroquine is highly effective agianst SARS-CoV-2 (COVID-19) infection with EC50 of 1.13 μM in Vero E6 cells. Chloroquine has anti-HIV-1 activity.
in vitro

Chloroquine is a widely used autophagy inhibitor.[5]
in vivo

The inhibition of autophagy by chloroquine potentiates the therapeutic effect of PI3K/AKT inhibitors in vitro and in vivo TNBC models, synergizing with taxane-based chemotherapy.[6]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HL‐60 cells
濃度 10 µM
反応時間 48 h
実験の流れ

One day before treatment, AML cells were seeded with a density of 2 × 105 cells/mL. Fresh medium containing different concentrations of Ara‐C was added into the culture medium. Cells were further cultured for 24 or 48 h based on experimental purposes. For inhibition of autophagy, HL‐60 cells were treated with 200 nM bafilomycin A1 or 10 µM chloroquine. Overexpression and knockdown were performed by transfection.

(Data sourced from selleck products)
動物実験 動物モデル Female NOD/SCID athymic mice injected with MDAMB231 cells
投薬量 30 mg/kg
投与方法 o.g.

(Data sourced from selleck products)

参考

  • https://pubmed.ncbi.nlm.nih.gov/25385822/
  • https://pubmed.ncbi.nlm.nih.gov/24273604/
  • https://pubmed.ncbi.nlm.nih.gov/32145363/
  • https://pubmed.ncbi.nlm.nih.gov/11166661/
  • https://pubmed.ncbi.nlm.nih.gov/33638615/
  • https://pubmed.ncbi.nlm.nih.gov/35761360/

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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