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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C17H8Cl3N3O2 |
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分子量 | 392.62 | CAS No. | 1370554-01-0 | |
Solubility (25°C)* | 体外 | DMSO | 20 mg/mL (50.93 mM) | |
Water | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Ciliobrevin D (compound 5) is a cell-permeable, reversible and specific antagonist of AAA+ (ATPases associated with diverse cellular activities) ATPase motor cytoplasmic dynein. Ciliobrevin D perturbs primary cilia formation and blocks Hedgehog (Hh) signaling. |
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in vitro | Ciliobrevin D inactivate dynein in Sertoli cells. The inactivation of dynein by ciliobrevin D also perturbs gross disruption of F-actin across the Sertoli cells in vitro.[2] Ciliobrevin D perturbs protein trafficking within the primary cilium, leading to their malformation and Hedgehog signaling blockade. Ciliobrevin D also prevents spindle pole focusing, kinetochore-microtubule attachment, melanosome aggregation, and peroxisome motility in cultured cells.[1] |
in vivo | Ciliobrevin D inactivate dynein in the testis. The inactivation of dynein by ciliobrevin D also perturbs gross disruption of F-actin across the seminiferous epithelium in vivo, illustrating there are cross talks between the two cytoskeletons in the testis.[2] |
細胞アッセイ | 細胞株 | Sertoli cells |
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濃度 | 15 µM, 30 µM | |
反応時間 | 1 h | |
実験の流れ | Sertoli cells cultured in vitro on Matrigel-coated bicameral units at 1.0 × 106 cells/cm2 are used for quantifying the transepithelial electrical resistance in ohms (Ω) across the cell epithelium to assess the TJ-barrier function. In brief, each bicameral unit is placed inside the well of a 24-well dish with 0.5 ml F12/DMEM in the apical and the basal compartments. Dynein is inhibited in Sertoli cells on days 3 and 4 using Ciliobrevin D at 15 µM or 30 µM for 1hr, and Sertoli cell TJ-permeability barrier function is monitored daily by quantifying transepithelial electrical resistance across the cell epithelium. |
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動物実験 | 動物モデル | adult male Sprague-Dawley rats |
投薬量 | 15 µM per testis | |
投与方法 | local injection |
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Localization of a TORC1-eIF4F translation complex during CD8+ T cell activation drives divergent cell fate [ Mol Cell, 2022, S1097-2765(22)00327-6] | PubMed: 35597236 |
LRRK1-mediated NDEL1 phosphorylation promotes cilia disassembly via dynein-2-driven retrograde intraflagellar transport [ J Cell Sci, 2022, 135(21)jcs259999] | PubMed: 36254578 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。