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Synonyms | Limonene, Dipentene, Cyclohexene, Eulimen| | Storage (From the date of receipt) |
2 years -80°C liquid | |
化学式 | C10H16 |
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分子量 | 136.23 | CAS No. | 138-86-3 | |
Solubility (25°C)* | 体外 | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Cinene (Limonene, Dipentene) is a clear, colorless liquid hydrocarbon classified as a cyclic monoterpene, and is the major component in oil of citrus fruit peels. It is usually used to make fragrances and flavors.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing. |
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in vitro | Cinene (Limonene, Dipentene) treatment significantly elevates collagen synthesis, alkaline phosphatase activity, osteocalcin synthesis, and mineralization in osteoblastic cells. Limonene decreases interleukin (IL)-1 β-induced NF-κB, JNK and p38 activation and the expression of inflammatory (iNOS) and catabolic (MMP-1 and MMP-13) genes, while increasing the activation of anti-catabolic genes (TIMP-1) and ERK1/2 relative to IL-1β-treated cells in a cellular model of osteoarthritis. Limonene effectively protectes human lymphatic endothelial cells from H2O2-induced oxidative stress, and that its antioxidant effect is significant, increasing cell survival rate[1]. Limonene could inhibit LPS-induced production of nitric oxide, prostaglandin E2, and proinflammatory cytokines in RAW264.7 macrophages[2]. |
in vivo | In a mouse model of acute lung injury, limonene pretreatment at doses of 25, 50, and 75 mg/kg decreases LPS-induced evident lung histopathological changes, lung wet-to-dry weight ratio, and lung myeloperoxidase activity. In addition, pretreatment with limonene inhibits in flammatory cells and proin flammatory cytokines including tumor necrosis factor-α, interleukin-1β, and interleukin-6 in BALF (bronchoalveolar lavage fluid). Limonene blocks the phosphorylation of IκBα, nuclear factor-κB (NF-κB) p65, p38 mitogen-activated protein kinase (MAPK), c-Jun NH2-terminal kinase, and extracellular signal-regulated kinase in LPS-induced acute lung injury. It improves the pulmonary functions[2]. |
細胞アッセイ | 細胞株 | MC3T3-E1 osteoblastic cells |
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濃度 | 0.01-1 μM | |
反応時間 | 3 days | |
実験の流れ | MC3T3-E1 cells are cultured at 37°C in a 5% CO2 atmosphere in α-MEM. The media contained 10% heat-inactivated FBS, 100 U/ml penicillin and 100 mg/ml streptomycin. The cells are treated at confluence with culture media containing 5 mM β-glycerophosphate and 50 μg/ml ascorbic acid to initiate differentiation. After 3 days (for collagen content and alkaline phosphatase (ALP) activity) or 14 days (for osteocalcin and calcium deposition assay), the cells are incubated with limonene for 3 days. |
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動物実験 | 動物モデル | BALB/c mice |
投薬量 | 25, 50 and 75 mg/kg | |
投与方法 | i.p. |
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長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。