Cisplatin

製品コードS1166 バッチS116617

印刷

化学情報

Chemical Structure Synonyms NSC 119875, Cisplatinum, cis-diamminedichloroplatinum II, CDDP, cis DDP, DDP Storage
(From the date of receipt)		 2 years 4°C(in the dark) powder
化学式

Cl2H6N2Pt
分子量 300.05 CAS No. 15663-27-1
Solubility (25°C)* 体外 DMF 10 mg/mL (33.32 mM)
Water (warmed with 50ºC water bath) 1 mg/mL (3.33 mM)
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMF 40%PEG300 5%Tween80 50%ddH2O

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 0.700mg/ml (2.33mM) Taking the 1 mL working solution as an example, add 50 μL 14 mg/ml clarified DMF stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 シスプラチン (Cisplatin (NSC 119875, Cisplatinum, cis-diamminedichloroplatinum II, CDDP, cis DDP, DDP)) は無機白金錯体であり、腫瘍細胞中において DNA 付加物を形成することにより DNA 合成を阻害する能力を有します。シスプラチンはフェロトーシス (ferroptosis) を活性化し、オートファジー (autophagy) を誘導します。溶液は都度調製してください。Solutions are unstable and should be fresh-prepared.DMSO is not recommended to dissolve platinum-based drugs, which can easily lead to drug inactivation.
in vitro

Cisplatin induces cytotoxic by interaction with DNA to form DNA adducts which activate several signal transduction pathways, including Erk, p53, p73, and MAPK, which culminates in the activation of apoptosis. [1]

This compound (30 μM) treated for 6 h induces an apparent activation of Erk in HeLa cells, which is sustained over the following 14 h period. It also shows an effective antineoplastic activity by inducing tumor cells death[2].

It displays ability to cause renal proximal tubular cell (RPTC) apoptosis, causing cell shrinkage, a 50-fold increase in caspase 3 activity, a 4-fold increase in phosphatidylserine externalization, and 5- and 15-fold increases in chromatin condensation and DNA hypoploidy, respectively. [4]

This chemical (800 μM) causes typical features of necrosis of RPTC after treatment for 4 hr. [5]
in vivo

Cisplatin has been demonstrated to be efficient in regression tumor growth in a wide variety of animal tumors models, including head and neck cancer xenografts, cervical squamous carcinoma xenografts, testicular carcinoma xenografts, ovarian cancer xenografts, breast carcinoma xenografts, colonic carcinoma, heterotransplanted hepatoblastoma, and so on. This compound (5 mg/kg) given weekly i.v. at the day 1 and 7 induces a tumor growth inhibition (GI) of 77.5% and 85.1% of the serous xenografts Ov.Ri(C) and OVCAR-3, respectively. [6]
特徴 One of the most widely used and most potent chemotherapeutic agents. This product is not recommended to be dissolved in dimethylsulfoxide (DMSO).[7]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Leukemia L1210/0 cells
濃度 7 μg/mL
反応時間 2 hours
実験の流れ

L1210/0 cells are maintained in an exponential suspension culture at 37 ℃ in a humidified atmosphere of 5% CO2 in McCoy's medium 5a (modified), supplemented with 15% calfserum, and Fungizone. L1210/0 cells are incubated in Cisplatin (7 μg/mL) for 2 hr at 37 ℃. To measure growth inhibition, the cells are centrifuged, washed once, resuspended in fresh medium at 30 × 103 to 50 × 103 cells/mL, and incubated for 3 days. Cell numbers are determined on a Coulter Counter. An aliquot of cells is diluted with an equal volume of 0.4% trypan blue. Viability is recorded as the percentage of cells that has excluded trypan blue. Cells incubated with this compound as above are also diluted into 0.1% agar and allowed to grow for 2 weeks when colonies are counted.
動物実験 動物モデル Female NMRI/Cpb (nuinu) mice
投薬量 5 mg/kg
投与方法 i.v.

参考

  • https://pubmed.ncbi.nlm.nih.gov/14576837/
  • https://pubmed.ncbi.nlm.nih.gov/10993883/
  • https://pubmed.ncbi.nlm.nih.gov/3395999/
  • https://pubmed.ncbi.nlm.nih.gov/12065694/
  • https://pubmed.ncbi.nlm.nih.gov/8967349/
  • https://pubmed.ncbi.nlm.nih.gov/1563830/
  • https://pubmed.ncbi.nlm.nih.gov/24812268/
  • https://pubmed.ncbi.nlm.nih.gov/28494534/

カスタマーフィードバック

Data from [Cancer Res, 2014, 74(1), 298-308]

Data from [PLoS One, 2013, 8(1), e54595]

, 2013, Dr. Edita Aksamitiene from Thomas Jefferson University

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

A single-cell atlas reveals immune heterogeneity in anti-PD-1-treated non-small cell lung cancer [ Cell, 2025, S0092-8674(25)00291-0] PubMed: 40147443
Cisplatin and temozolomide combinatorial treatment triggers hypermutability and immune surveillance in experimental cancer models [ Cancer Cell, 2025, S1535-6108(25)00223-5] PubMed: 40513578
A pancreatic cancer organoid biobank links multi-omics signatures to therapeutic response and clinical evaluation of statin combination therapy [ Cell Stem Cell, 2025, S1934-5909(25)00265-6] PubMed: 40812300
Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma [ Cancer Commun (Lond), 2025, 10.1002/cac2.70036] PubMed: 40443016
SUMO4 promotes SUMO deconjugation required for DNA double-strand-break repair [ Mol Cell, 2025, 85(5):877-893.e9] PubMed: 40054443
Organoid morphology-guided classification for oral cancer reveals prognosis [ Cell Rep Med, 2025, 6(5):102129] PubMed: 40359934
A Rac-specific competitive inhibitor of guanine nucleotide binding reduces metastasis in triple-negative breast cancer [ Cell Rep Med, 2025, 6(7):102233] PubMed: 40633540
TBK1 is a signaling hub in coordinating stress-adaptive mechanisms in head and neck cancer progression [ Autophagy, 2025, 1-23.] PubMed: 40114316
A novel pathway for stemness propagation and chemoresistance in non-small cell lung cancer via phosphorylated PKM2-loaded small extracellular vesicles [ Theranostics, 2025, 15(8):3439-3461] PubMed: 40093893
Identification of microprotein-coding intronic polyadenylation isoforms and function in genotoxic anticancer drug response [ Genome Biol, 2025, 26(1):366] PubMed: 41131620

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人間や獣医の診断であるか治療的な使用のためにでない。

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