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Synonyms | CK-0944636 | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C16H16N2OS |
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分子量 | 284.38 | CAS No. | 442632-72-6 | |
Solubility (25°C)* | 体外 | DMSO | 57 mg/mL (200.43 mM) | |
Ethanol | 50 mg/mL (175.82 mM) | |||
Water | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | CK-636 (CK-0944636) is an Arp2/3 complex inhibitor with IC50 of 4 μM, 24 μM and 32 μM for inhibition of actin polymerization induced by human, fission yeast and bovine Arp2/3 complex, respectively. |
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in vitro | CK-636 inhibits actin polymerization mediated by Arp2/3 complex in live cells, and reduces the formation of actin filament comet tails by Listeria in infected SKOV3 cells. [1] CK-636 inhibits lamellipodia formation at the leading edges of migrating T cells, and causes reduced velocity. In addition, a substantial fraction of CK-636-treated T cells on zigzag patterns with an acute turning angle are trapped near the interfaces formed by the turning points of zigzag patterns. [2] |
キナーゼアッセイ | Etheno-ATP fluorescent assay | |
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Human Arp2/3 complex (0.7 μM) is incubated with 2.5 μM etheno-ATP, 0.2 M acylamide (used as a etheno-ATP fluorescence quencher) in 2mM Trs-HCl pH 8.0, 50 mM KCl, 0.05 mM EGTA 0.8 mM MgCl2, 0 and 0.5 mM DTT. Ethano-ATP emission spectrums are acquired upon excitation at 340 nm using spectrofluorometer FluoroMax. Inhibition of Arp2/3 complex by 100 μM CK-636 is performed in the presence or absence of 2.8 μM WASp-VCA. |
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Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors [ Elife, 2021, 10e65910] | PubMed: 34796874 |
A self-enforcing HOXA11/Stat3 feedback loop promotes stemness properties and peritoneal metastasis in gastric cancer cells. [ Theranostics, 2019, 9(25):7628-7647] | PubMed: 31695791 |
Intracellular Trafficking Pathways of Edwardsiella tarda: From Clathrin- and Caveolin-Mediated Endocytosis to Endosome and Lysosome. [ Front Cell Infect Microbiol, 2017, 7:400] | PubMed: 28932708 |
Actin cytoskeleton regulator Arp2/3 complex is required for DLL1 activating Notch1 signaling to maintain the stem cell phenotype of glioma initiating cells [ Oncotarget, 2017, 8(20):33353-33364] | PubMed: 28380416 |
Actin cytoskeleton regulator Arp2/3 complex is required for DLL1 activating Notch1 signaling to maintain the stem cell phenotype of glioma initiating cells. [ Oncotarget, 2017, 8(20):33353-33364] | PubMed: 28380416 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。