Datasheet

生物学的記述

Specificity	Cleaved PARP (Asp214) Antibody [C3D4] detects endogenous levels of the large fragment (89 kDa) of PARP protein only when cleaved at Asp214.
Background	Cleaved PARP (Asp214) is the caspase-3-generated cleavage product of poly(ADP-ribose) polymerase 1 (PARP-1), a nuclear enzyme central to DNA repair. PARP-1 consists of an N-terminal DNA-binding domain with zinc fingers (residues 1–214), a central automodification domain, and a C-terminal catalytic domain. During apoptosis, caspase-3 cleaves PARP-1 specifically between Asp214 and Gly215, resulting in a 24 kDa DNA-binding fragment and an 89 kDa catalytic fragment. This cleavage inactivates PARP-1’s DNA repair capacity by disrupting DNA binding and poly(ADP-ribosyl)ation activity, thereby preventing NAD+ depletion, limiting necrosis, and promoting orderly apoptotic progression. The presence of cleaved PARP is widely used as a hallmark of apoptosis, signaling caspase activation and cellular disassembly.

Specificity

Cleaved PARP (Asp214) Antibody [C3D4] detects endogenous levels of the large fragment (89 kDa) of PARP protein only when cleaved at Asp214.

Background

Cleaved PARP (Asp214) is the caspase-3-generated cleavage product of poly(ADP-ribose) polymerase 1 (PARP-1), a nuclear enzyme central to DNA repair. PARP-1 consists of an N-terminal DNA-binding domain with zinc fingers (residues 1–214), a central automodification domain, and a C-terminal catalytic domain. During apoptosis, caspase-3 cleaves PARP-1 specifically between Asp214 and Gly215, resulting in a 24 kDa DNA-binding fragment and an 89 kDa catalytic fragment. This cleavage inactivates PARP-1’s DNA repair capacity by disrupting DNA binding and poly(ADP-ribosyl)ation activity, thereby preventing NAD+ depletion, limiting necrosis, and promoting orderly apoptotic progression. The presence of cleaved PARP is widely used as a hallmark of apoptosis, signaling caspase activation and cellular disassembly.

使用情報

Application

WB, IP, IHC, IF, FCM

Dilution

WB	IP	IHC	IF	FCM
1:1000	1:100	1:100	1:800	1:100

Reactivity

Mouse, Rat

Source

Rabbit Monoclonal Antibody

MW

89 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/11907276/
https://pubmed.ncbi.nlm.nih.gov/10373561/

Application Data

WB

Validated by Selleck

Lane 1: Neuro-2a (serum starvation treated), Lane 2: Neuro-2a (serum starvation treated; Staurosporine, 1 μM, 3 h)