受注:045-509-1970 |
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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C(in the dark) powder | |
化学式 | C26H21N3O3 |
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分子量 | 423.46 | CAS No. | 55779-48-1 | |
Solubility (25°C)* | 体外 | DMSO | 84 mg/mL (198.36 mM) | |
Ethanol | 1 mg/mL (2.36 mM) | |||
Water | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Coelenterazine is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. Renilla luciferase and substrate coelenterazine have been used as bioluminescence donors in bioluminescence resonance energy transfer (BRET) to detect protein-protein interactions. Coelenterazine is a superoxide anion-sensitive chemiluminescent probe, and it can also be used in the chemiluminescent detection of peroxynitrite. | |||||
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in vitro | When monitoring various biological processes, coelenterazine (CTZ) can be catalyzed by luciferases, such as the sea pansy Renilla reniformis (Rluc) and the marine copepod Gaussia princeps (Gluc), which results in emission of blue light. In Gli36-Gluc- or Gli36-Rluc-expressing Gli36 cells, coelenterazine (8 礸/ml) shows a bioluminescence signal. [1] In RIN-5F, 4T1, HCT 116, HEK 293, 293FT, HeLa and INS-1 cells, coelenterazine can be used to assay superoxide anion concentrations. [2] |
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in vivo | In 4T1-implanted mouse, Coelenterazine detects cancer-associated superoxide anion and used to evaluate the role of superoxide anion in cancer cell biology and its role in pathology. [2] |
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密度 | 1.32 g/mL |
RNAi-mediated rheostat for dynamic control of AAV-delivered transgenes [ Nat Commun, 2023, 14(1):1970] | PubMed: 37031257 |
The molecular evolution of C4-associated gene regulation in the genus Flaveria [ The UWA Research Repository, 2023, ] | PubMed: none |
Exendin-4 gene modification and microscaffold encapsulation promote self-persistence and antidiabetic activity of MSCs [ Sci Adv, 2021, 7(27)eabi4379] | PubMed: 34215590 |
Development of a Bioassay for Continuous Monitoring of TWIST1 Activity [ Preprints, 2021, 10.20944/preprints202103.0265.v1] | PubMed: N/A |
A novel bioassay for quantification of surface Cannabinoid receptor 1 expression [ Sci Rep, 2020, 10(1):18191] | PubMed: 33097803 |
Tumor treating fields increases membrane permeability in glioblastoma cells [ Cell Death Discov, 2018, 4:113] | PubMed: 30534421 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。