受注:045-509-1970 |
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Synonyms | Colchineos, Colchisol, Colcin,NSC 757 | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C22H25NO6 |
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分子量 | 399.44 | CAS No. | 64-86-8 | |
Solubility (25°C)* | 体外 | DMSO | 79 mg/mL (197.77 mM) | |
Water | 79 mg/mL (197.77 mM) | |||
Ethanol | 79 mg/mL (197.77 mM) | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Colchicine is a microtubule polymerization inhibitor with an IC50 of 3 nM. |
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in vitro | Colchicine is a tubulin inhibitor and a microtubule disrupting agent, which inhibits the tubulin polymerization on the site of β-tubulin.[1] |
in vivo | Colchicine prevents NSAID-induced small intestinal injury by inhibiting activation of the NLR family pyrin domain-containing 3 (NLRP3) inflammasome.[2] |
細胞アッセイ | 細胞株 | HeLa cells |
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濃度 | 5 μM | |
反応時間 | 2 h | |
実験の流れ | HeLa cells are cultured in 6-well plates and then treated with or without different concentrations of KXO1, vinblastine, or colchicine for 2 h before being treated with 100 μM EBI for another 2 h. Total protein is extracted with radioimmuno_x0002_precipitation assay buffer and subjected to Western blot analy_x0002_sis of β-tubulin. GAPDH is used as a loading control. The Western blotting procedure is performed. |
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動物実験 | 動物モデル | Specific-pathogen-free 8-week-old male mice, Wild-type C57BL/6 mice, NLRP3−/− mice |
投薬量 | 1 or 3mg/kg | |
投与方法 | o.g. |
Novel Brain-Penetrant, Small-Molecule Tubulin Destabilizers for the Treatment of Glioblastoma [ Biomedicines, 2024, 12(2)406] | PubMed: 38398008 |
Restricting epigenetic activity promotes the reprogramming of transformed cells to pluripotency in a line-specific manner [ Cell Death Discov, 2023, 9(1):245] | PubMed: 37452056 |
DNA nicks induce mutational signatures associated with BRCA1 deficiency [ Nat Commun, 2022, 13(1):4285] | PubMed: 35879372 |
DNA nicks induce mutational signatures associated with BRCA1 deficiency [ Nat Commun, 2022, 13(1):4285] | PubMed: 35879372 |
Intravital Imaging Reveals the Ameliorating Effect of Colchicine in a Photothrombotic Stroke Model via Inhibition of Neutrophil Recruitment [ Transl Stroke Res, 2022, 10.1007/s12975-022-01022-7] | PubMed: 35441983 |
Colchicine Ameliorates Dilated Cardiomyopathy Via SIRT2-Mediated Suppression of NLRP3 Inflammasome Activation [ J Am Heart Assoc, 2022, 11(13):e025266] | PubMed: 35766262 |
IMB5476, a novel microtubule inhibitor, induces mitotic catastrophe and overcomes multidrug resistance in tumors [ Eur J Pharmacol, 2022, 919:174802] | PubMed: 35143830 |
Colchicine alleviates inflammation and improves diastolic dysfunction in heart failure rats with preserved ejection fraction [ Eur J Pharmacol, 2022, 929:175126] | PubMed: 35779623 |
Alpha-synuclein/MPP+ mediated activation of NLRP3 inflammasome through microtubule-driven mitochondrial perinuclear transport [ Biochem Biophys Res Commun, 2022, 594:161-167] | PubMed: 35085893 |
Reciprocal interaction between SIRT6 and APC/C regulates genomic stability [ Sci Rep, 2021, 11(1):14253] | PubMed: 34244565 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。