Colchicine

製品コードS2284 バッチS228405

化学情報

	Synonyms	Colchineos, Colchisol, Colcin,NSC 757	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₂₂H₂₅NO₆
	分子量	399.44	CAS No.	64-86-8
Solubility (25°C)*	体外	DMSO	80 mg/mL (200.28 mM)
		Ethanol	80 mg/mL (200.28 mM)
		Water	79 mg/mL (197.77 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Colchicine is a microtubule polymerization inhibitor with an IC50 of 3 nM.
in vitro	Colchicine is a tubulin inhibitor and a microtubule disrupting agent, which inhibits the tubulin polymerization on the site of β-tubulin.^[1]
in vivo	Colchicine prevents NSAID-induced small intestinal injury by inhibiting activation of the NLR family pyrin domain-containing 3 (NLRP3) inflammasome.^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HeLa cells
	濃度	5 μM
	反応時間	2 h
	実験の流れ	HeLa cells are cultured in 6-well plates and then treated with or without different concentrations of KXO1, vinblastine, or colchicine for 2 h before being treated with 100 μM EBI for another 2 h. Total protein is extracted with radioimmuno_x0002_precipitation assay buffer and subjected to Western blot analy_x0002_sis of β-tubulin. GAPDH is used as a loading control. The Western blotting procedure is performed.
動物実験	動物モデル	Specific-pathogen-free 8-week-old male mice, Wild-type C57BL/6 mice, NLRP3^−/− mice
	投薬量	1 or 3mg/kg
	投与方法	o.g.

参考

[1] Niu L, et al. J Biol Chem. 2019 Nov 29;294(48):18099-18108.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Novel Brain-Penetrant, Small-Molecule Tubulin Destabilizers for the Treatment of Glioblastoma [ Biomedicines, 2024, 12(2)406]	PubMed: 38398008
Restricting epigenetic activity promotes the reprogramming of transformed cells to pluripotency in a line-specific manner [ Cell Death Discov, 2023, 9(1):245]	PubMed: 37452056
DNA nicks induce mutational signatures associated with BRCA1 deficiency [ Nat Commun, 2022, 13(1):4285]	PubMed: 35879372
DNA nicks induce mutational signatures associated with BRCA1 deficiency [ Nat Commun, 2022, 13(1):4285]	PubMed: 35879372
Intravital Imaging Reveals the Ameliorating Effect of Colchicine in a Photothrombotic Stroke Model via Inhibition of Neutrophil Recruitment [ Transl Stroke Res, 2022, 10.1007/s12975-022-01022-7]	PubMed: 35441983
Colchicine Ameliorates Dilated Cardiomyopathy Via SIRT2-Mediated Suppression of NLRP3 Inflammasome Activation [ J Am Heart Assoc, 2022, 11(13):e025266]	PubMed: 35766262
IMB5476, a novel microtubule inhibitor, induces mitotic catastrophe and overcomes multidrug resistance in tumors [ Eur J Pharmacol, 2022, 919:174802]	PubMed: 35143830
Colchicine alleviates inflammation and improves diastolic dysfunction in heart failure rats with preserved ejection fraction [ Eur J Pharmacol, 2022, 929:175126]	PubMed: 35779623
Alpha-synuclein/MPP+ mediated activation of NLRP3 inflammasome through microtubule-driven mitochondrial perinuclear transport [ Biochem Biophys Res Commun, 2022, 594:161-167]	PubMed: 35085893
Reciprocal interaction between SIRT6 and APC/C regulates genomic stability [ Sci Rep, 2021, 11(1):14253]	PubMed: 34244565

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。