Colchicine

製品コードS2284 バッチS228406

印刷

化学情報

Synonyms

Colchineos, Colchisol, Colcin,NSC 757

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₂H₂₅NO₆

分子量

399.44

CAS No.

64-86-8

Solubility (25°C)*

体外

DMSO

80 mg/mL (200.28 mM)

Water

80 mg/mL (200.28 mM)

Ethanol

80 mg/mL (200.28 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	4.000mg/ml (10.01mM)	Taking the 1 mL working solution as an example, add 50 μL of 80 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.400mg/ml (1.00mM)	Taking the 1 mL working solution as an example, add 50 μL of 8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Colchicine is a microtubule polymerization inhibitor with an IC50 of 3 nM.
in vitro	Colchicine is a tubulin inhibitor and a microtubule disrupting agent, which inhibits the tubulin polymerization on the site of β-tubulin.^[1]
in vivo	Colchicine prevents NSAID-induced small intestinal injury by inhibiting activation of the NLR family pyrin domain-containing 3 (NLRP3) inflammasome.^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HeLa cells
	濃度	5 μM
	反応時間	2 h
	実験の流れ	HeLa cells are cultured in 6-well plates and then treated with or without different concentrations of KXO1, vinblastine, or colchicine for 2 h before being treated with 100 μM EBI for another 2 h. Total protein is extracted with radioimmuno_x0002_precipitation assay buffer and subjected to Western blot analy_x0002_sis of β-tubulin. GAPDH is used as a loading control. The Western blotting procedure is performed.
動物実験	動物モデル	Specific-pathogen-free 8-week-old male mice, Wild-type C57BL/6 mice, NLRP3^−/− mice
	投薬量	1 or 3mg/kg
	投与方法	o.g.

参考

https://pubmed.ncbi.nlm.nih.gov/31628188/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Cucurbitacin B Exhibits Antitumor Effects on Chordoma Cells via Disruption of Brachyury [ Int J Mol Sci, 2025, 26(8)3864]	PubMed: 40332566
Dual inhibition of topoisomerase II and microtubule of podophyllotoxin derivative 5p overcomes cancer multidrug resistance [ Eur J Pharmacol, 2024, 983:176968]	PubMed: 39233039
Novel Brain-Penetrant, Small-Molecule Tubulin Destabilizers for the Treatment of Glioblastoma [ Biomedicines, 2024, 12(2)406]	PubMed: 38398008
Endothelial cell elongation and alignment in response to shear stress requires acetylation of microtubules [ Front Physiol, 2024, 15:1425620]	PubMed: 39318362
MI-181 Modulates Cilia Length and Restores Cilia Length in Cells with Defective Shortened Cilia [ ACS Chem Biol, 2024, 19(8):1733-1742]	PubMed: 39106364
Restricting epigenetic activity promotes the reprogramming of transformed cells to pluripotency in a line-specific manner [ Cell Death Discov, 2023, 9(1):245]	PubMed: 37452056
Understanding the Role of Pancreatic Ductal Adenocarcinoma Chemoresistance and Intertumoral Heterogeneity on Vesicular Stomatitis Virus-Based Oncolytic [ ProQuest, 2023, 30572078]	PubMed: None
DNA nicks induce mutational signatures associated with BRCA1 deficiency [ Nat Commun, 2022, 13(1):4285]	PubMed: 35879372
DNA nicks induce mutational signatures associated with BRCA1 deficiency [ Nat Commun, 2022, 13(1):4285]	PubMed: 35879372
Intravital Imaging Reveals the Ameliorating Effect of Colchicine in a Photothrombotic Stroke Model via Inhibition of Neutrophil Recruitment [ Transl Stroke Res, 2022, 10.1007/s12975-022-01022-7]	PubMed: 35441983

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。