Colivelin

製品コードS9664 バッチS966401

印刷

化学情報

Synonyms

CLN

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₁₁₉H₂₀₆N₃₂O₃₅

分子量

2645.10

CAS No.

867021-83-8

Solubility (25°C)*

体外

Water

100 mg/mL (37.8 mM)

体内（毎回新しく調製した物を用意してください）

Clear solution

5% H2O 1 40% PEG 300 2 5% Tween 80 3 ddH2O

5.0mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified H2O stock solution to 400 μL of PEG 300, mix evenly to clarify; add 50 μL of Tween 80 to the above system, mix evenly to clarify; Then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Colivelin (CLN) is a brain-penetrant neuroprotective peptide with potent long-term capacity against Aβ deposition, neuronal apoptosis, and synaptic plasticity deficits in neurodegenerative disease. Colivelin is an activator of STAT3.
in vitro	Colivelin, a brain penetrant neuroprotective peptide, significantly increases the p-STAT3 protein levels in BV-2 cells.^[2]
in vivo	Colivelin protects against ischemic brain injury and improves neurological outcomes, by elevating anti-apoptotic genes up-regulation and activates JAK/STAT3 signaling afetr ischemic stroke.^[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	BV-2 cells
	濃度	50 µg/ml
	反応時間	4 h
	実験の流れ	BV-2 cells are maintained at 37℃ with 5% CO₂. To build OGD models, BV-2 cells are washed with PBS for 3 times, and cultured in glucose-free DMEN and placed in a hypoxia incubation chamber filled with the gas mixture for 4 h at 37℃. The culture medium is then replaced by the normal medium containing 4.5 g/L glucose and cells are cultured under normoxic conditions for reoxygenation for 24 h. BV-2 cells are treated with 50 µg/ml rmMFG-E8 or/and Colivelin TFA, respectively. The expression changes of proteins (p-STAT3 and SOCS3) are analyzed by Western blot assay.
動物実験	動物モデル	Male C57BL/6 mice of middle cerebral artery occlusion (MCAO) models
	投薬量	1 mg/kg
	投与方法	i.p.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Intracellular CIRP promotes liver regeneration while extracellular CIRP induces ER stress after partial hepatectomy in mice [ Research Square, 2024, 10.21203/rs.3.rs-3840462/v1]	PubMed: none
TIPE1 inhibits osteosarcoma tumorigenesis and progression by regulating PRMT1 mediated STAT3 arginine methylation [ Cell Death Dis, 2022, 13-9:815]	PubMed: 36151091
IL-27 promotes decidualization via the STAT3-ESR/PGR regulatory axis [ J Reprod Immunol, 2022, 151:103623]	PubMed: 35430461
Complement C5a induces the generation of neutrophil extracellular traps by inhibiting mitochondrial STAT3 to promote the development of arterial thrombosis [ Thromb J, 2022, 20(1):24]	PubMed: 35488279
HCRP-1 alleviates the malignant phenotype and angiogenesis of oral squamous cell carcinoma cells via the downregulation of the EGFR/STAT3 signaling pathway [ Oncol Lett, 2022, 24(5):387]	PubMed: 36276496
Shikonin alleviates choroidal neovascularization by inhibiting proangiogenic factor production from infiltrating macrophages [ Exp Eye Res, 2021, 213:108823]	PubMed: 34752817

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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