Corticosterone

製品コードS4752 バッチS475203

印刷

化学情報

Chemical Structure Synonyms 17-deoxycortisol, 11β,21-dihydroxyprogesterone, NSC-9705 Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C21H30O4
分子量 346.46 CAS No. 50-22-6
Solubility (25°C)* 体外 DMSO 69 mg/mL (199.15 mM)
Ethanol (warmed with 50ºC water bath) 9 mg/mL (25.97 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Corticosterone, the major stress hormone, is an adrenocortical steroid that has modest but significant activities as a mineralocorticoid and a glucocorticoid.
in vitro Corticosterone, via SGK phosphorylation of GDI at Ser-213, increases the formation of GDI-Rab4 complex, facilitating the functional cycle of Rab4 and Rab4-mediated recycling of AMPARs to the synaptic membrane. It enhances AMPAR-mediated miniature excitatory postsynaptic current (mEPSC) amplitude and surface expression of GluR1 and GluR2 subunits in hippocampal neurons, increases the surface mobility and synaptic content of AMPAR GluR2 subunits, and enhances L-type calcium currents in CA1 pyramidal neurons. Corticosterone operates through mineralocorticoid receptors and glucocorticoid receptors, both of which belong to the family of nuclear receptors that bind to response elements in the DNA, thus modifying the activity of responsive genes[1].
in vivo Corticosterone plays an important role in regulating neuronal functions of the limbic system. After stress, the level of stress hormones such as corticosterone is markedly increased. Corticosterone exerts a time- and region-specific action on cellular physiology of limbic neurons[1].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HEK293 cells
濃度 100 nM
反応時間 30 min
実験の流れ HEK293 cells are grown in 6-cm dishes in 10% fetal bovine serum DMEM medium. When cells are 90% confluent, the medium is changed to 0.5% fetal bovine serum DMEM to limit serum-induced up-regulation of SGK. For in vitro phosphorylation analysis, the following approach is used. HEK293 cells are transfected with or without SGK1 small interfering RNA. One day after transfection, cells are treated without or with 100 nM corticosterone for 30 min, washed, and maintained in 0.5% fetal bovine serum DMEM for 1.5 h. Then cells are lysed in the CytoBuster protein extraction reagent containing protease inhibitors. Cell lysates are centrifuged at 16,000 × g at 4 °C for 20 min. The supernatants (40 μl, ∼50 μg of total protein) are incubated with 1 μg of purified GST fusion protein of wild-type GDI or its mutants for 30 min at 30 °C in the reaction buffer (30 mM HEPES, pH 7.5, 10 mM MgCl2, 30 μM ATP, 1 μCi of [γ-32P]ATP, 100 nM calyculin, 1 μM okadaic acid). SDS-PAGE is carried out, and phosphorylated GDI is visualized with autoradiography.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

MicroRNA-451a is a candidate biomarker and therapeutic target for major depressive disorder [ Gen Psychiatr, 2024, 37(1):e101291] PubMed: 38304710
Novel Estrogen Receptor Dimerization BRET-Based Biosensors for Screening Estrogenic Endocrine-Disrupting Chemicals [ Biomater Res, 2024, 28:0010] PubMed: 38464469
Androgens improve ovarian follicle function impaired by glucocorticoids through an androgen-IGF1-FSH synergistic effect [ Front Endocrinol -Lausanne), 2022, 13:951928] PubMed: 36339442
Vocal babbling in a wild parrot shows life history and endocrine affinities with human infants [ Proc Biol Sci, 2022, 289(1976):20220592] PubMed: 35642373
miR-182 mediated the inhibitory effects of NF-κB on the GPR39/CREB/BDNF pathway in the hippocampus of mice with depressive-like behaviors [ Behav Brain Res, 2021, 418:113647] PubMed: 34743948
Overexpression of SIRT1 Inhibits Corticosterone-Induced Autophagy [ Neuroscience, 2019, 411:11-22] PubMed: 31146010
Impacts of ABCB1 (G1199A) polymorphism on resistance, uptake, and efflux to steroid drugs. [Peng R, et al. Xenobiotica, 2016, 46(10):948-52] PubMed: 26822676

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。