Cyanidin Chloride

製品コードS4916

印刷

化学情報

Chemical Structure Synonyms Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C15H11O6.Cl
分子量 322.7 CAS No. 528-58-5
Solubility (25°C)* 体外  
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

生物活性

製品説明 果物や野菜に豊富に含まれる天然フラボノイドであるCyanidin Chlorideは、肝臓X受容体アルファおよびベータのアゴニストリガンドであり、それぞれ3.5 μMおよび125.2 μMのEC50値を示します。
in vitro Cyanidin, a natural flavonoid abundant in fruits and vegetables, is known to regulate cellular lipid metabolism. Cyanidin induces the transactivation of Liver X receptors (LXRs) and binds directly to the ligand-binding domain of both LXRα and LXRβ with dissociation constants (KD) of 2.2 and 73.2 μM, respectively. Cell-free fluorescence energy transfer (FRET) analysis demonstrates that cyanidin induces the recruitment of co-activator peptide for LXRa and LXRb with EC50 of 3.5 μM and 125.2 μM, respectively. Cyanidin reduces intracellular cholesterol and triglyceride concentrations in both THP-1-derived macrophages and HepG2 cells. In addition, Cyanidin-3-glucoside, a glucoside derivative of cyanidin, is found to be a inhibitor of Fyn kinase, which inhibits Benzo[a]pyrene-7,8-diol-9,10-epoxide (B[a]PDE)-induced activation of AP-1 and NF-kB and attenuates the phosphorylation of MEK, MKK4, Akt, and MAPKs in JB6 P+ mouse epidermal cells.
in vivo Male C57BL/6J obese mice feed a high-fat diet for 12 weeks and genetically diabetic db/db mice at an age of 6 weeks receive dietary cyanidin-3-glucoside supplementation (0.2%) for 5 weeks. It shows that dietary cyanidin-3-glucoside lowers fasting glucose levels and markedly improves the insulin sensitivity in both high-fat diet fed and db/db mice .

プロトコル(参考用のみ)

キナーゼアッセイ TR-FRET co-activator assay
Increasing concentrations of cyanidin are added to the ligand binding domain (LBD) of LXR proteins and the co-activator peptides: fluorescein-TRAP220/DRIP-2 for LXR&alpha and fluorescein-D22 for LXR&beta with DMSO (1%) used as a vehicle. After 2 h incubation at room temperature, the 520/495 TR-FRET ratio is measured using a Spectra Max instrument with TRF laser excitation. Binding curves are generated by plotting the emission ratio vs. the log[cyanidin].
細胞アッセイ 細胞株 THP-1-derived macrophages and HepG2 cells
濃度 5, 10, 50, or 100 μM
反応時間 24 h
実験の流れ THP-1-derived macrophages or HepG2 cells are incubated with free fatty acids (palmitic acid and oleic acid) for 24 h. Then, both cells are treated with cyanidin (5, 10, 50, or 100 μM) for a further 24 h with DMSO (1%) as vehicle. Lipid extraction, intracellular total cholesterol, and triglyceride concentrations are quantified using a Cobas C111 automatic analyzer.
動物実験 動物モデル High-fat diet-fed and db/db mice
投薬量
投与方法 Orally

参考

  • https://pubmed.ncbi.nlm.nih.gov/23769638/
  • https://pubmed.ncbi.nlm.nih.gov/21501596/
  • https://pubmed.ncbi.nlm.nih.gov/21543211/

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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