Cycloastragenol

製品コードS3894 バッチS389403

印刷

化学情報

 Chemical Structure Synonyms CAG, TA-65, Cyclogalegigenin, Astramembrangenin Storage
(From the date of receipt)
3 years -20°C powder
1 years -80°C in solvent
化学式

C30H50O5

分子量 490.72 CAS No. 84605-18-5
Solubility (25°C)* 体外 DMSO 98 mg/mL (199.7 mM)
Ethanol 25 mg/mL (50.94 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

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生物活性

製品説明 Cycloastragenol (CAG, TA-65, Cyclogalegigenin, Astramembrangenin) is a saponin comprising a group of oil glucosides naturally present in a number of plants. It is a potent telomerase activator in neuronal cells.
in vitro Cycloastragenol stimulates telomerase activity and cell proliferation in human neonatal keratinocytes. It induces telomerase activity and cAMP response element binding (CREB) activation in PC12 cells and primary neurons. CAG treatment not only induces the expression of bcl2, a CREB-regulated gene, but also the expression of telomerase reverse transcriptase in primary cortical neurons[1]. Cycloastragenol rapidly passes through the Caco-2 cell monolayer by passive diffusion, once passage through the intestinal epithelium, first-pass intestinal metabolism of Cycloastragenol might occur. Cycloastragenol can undergo extensive metabolism in rat and human liver microsomes[2].
in vivo Oral administration of cycloastragenol (CA) for 7 days attenuates depression-like behavior in experimental mice. Oral bioavailability of cycloastragenol is about 25.70% at 10 mg/kg. Cycloastragenol is excreted through bile and feces and eliminated predominantly by the kidney in rats. It also might exist an enterohepatic circulation of cycloastragenol in rats. Cycloastragenol could be metabolized widely in vivo in rat. For oral administration the mean Tmax at 10 mg/kg, 20 mg/kg, and 40 mg/kg is 2.06±0.58 h, 1.48±0.36 h, and 2.35± 1.17 h. The t1/2 is 5.23±1.55 h, 7.33±3.03 h, and 6.06± 3.42 h, respectively. The mean absorption time of CA at 10 mg/kg is 5.70± 1.62 h; the poor absorption could be caused by the low solubility. The pharmacokinetic parameters show no significant differences among the groups of 10, 20, and 40 mg/kg except for Cmax and AUC[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HEKn cells, PC12 cells
濃度 --
反応時間 24 h
実験の流れ HEKn cells (population doubling time: 3-6 days), PC12 cells (passage No.: 12-18), cultured primary cortical, and hippocampal neurons [12 days in vitro (DIV)] are treated with CAG for 24 h. Total cell lysates are then collected by using the lysis buffer provided in the real-time quantitative telomeric repeat amplification protocol (RQ-TRAP) assay kit. Telomerase activity is determined by using an ABI Prism 7000.
動物実験 動物モデル male ICR mice
投薬量 100 mg/kg
投与方法 by oral gavage

Selleckの高級品が、幾つかの出版された研究調査結果(以下を含む)で使われた:

Noncoding telomeric repeat-containing RNA inhibits the progression of hepatocellular carcinoma by regulating telomerase-mediated telomere length [ Cancer Sci, 2020, 111(8):2789-2802] PubMed: 32357278

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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