Cycloheximide

製品コードS7418 バッチS741805

印刷

化学情報

Chemical Structure Synonyms NSC-185, Actidione, Naramycin A, CHX, FT 3422-2, NM-MCD 80 Storage
(From the date of receipt)		 3 years -20°C(in the dark) powder
1 year -80°C(in the dark) in solvent
化学式

C15H23NO4
分子量 281.35 CAS No. 66-81-9
Solubility (25°C)* 体外 DMSO 56 mg/mL (199.04 mM)
Ethanol 56 mg/mL (199.04 mM)
Water 15 mg/mL (53.31 mM)
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Cycloheximide, an antifungal antibiotic, is an eukaryote protein synthesis inhibitor with IC50 of 532.5 nM and 2880 nM for protein synthesis and RNA synthesis in vivo, respectively. Cycloheximide suppresses ferroptosis and inhibits autophagy.Cycloheximide (NSC-185) can be used to induce animal models of Dysmnesia.Solutions are unstable and should be fresh-prepared.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
in vitro

Cycloheximide, a protein synthesis inhibitor, reduces GSIV-induced host cell death by attenuating phosphatidylserine exposure and loss of ΔΨm from apoptosis/necrosis.[1]
in vivo

This compound, a protein synthesis inhibitors, acts on memory by altering modulators of memory formation as a secondary consequence of the inhibition of protein synthesis.[2]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 grouper fin cell line (GF-1)
濃度 0.33 µg/ml
反応時間 0-5 days
実験の流れ

Monolayers of GF-1 cells (4.0 mL, 105 cells/mL) on 60-mm Petri dishes are cultivated for at least 20 h, rinsed twice with phosphate buffered saline (PBS), treated with the protein synthesis inhibitor cycloheximide (CHX, 0.33 µg/mL) and ANT inhibitor BKA (20 µM) for 0 5 days, then infected with GSIV K1 strain (multiplicity of infection [m.o.i. ] = 5) for 0 5 days post-infection (dpi). At the end of the incubation period, each sample is removed from the medium and washed with PBS. Cells are then incubated for 10-15 min with 100 µL staining solution.
動物実験 動物モデル Male ICR mice
投薬量 30mg/kg, 60mg/kg,120 mg/kg
投与方法 i.p.

参考

  • https://pubmed.ncbi.nlm.nih.gov/26548846/
  • https://pubmed.ncbi.nlm.nih.gov/22610049/
  • https://pubmed.ncbi.nlm.nih.gov/5541758/
  • https://pubmed.ncbi.nlm.nih.gov/30916348/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Effective targeting of PDGFRA-altered high-grade glioma with avapritinib [ Cancer Cell, 2025, S1535-6108(25)00070-4] PubMed: 40086436
Host FSTL1 defines the impact of stem cell therapy on liver fibrosis by potentiating the early recruitment of inflammatory macrophages [ Signal Transduct Target Ther, 2025, 10(1):81] PubMed: 40050288
VCP downstream metabolite glycerol-3-phosphate (G3P) inhibits CD8+T cells function in the HCC microenvironment [ Signal Transduct Target Ther, 2025, 10(1):26] PubMed: 39848960
NAP1L1 degradation by FBXW7 reduces the deubiquitination of HDGF-p62 signaling to stimulate autophagy and induce primary cisplatin chemosensitivity in nasopharyngeal carcinoma [ Mol Cancer, 2025, 24(1):152] PubMed: 40414865
NAP1L1 degradation by FBXW7 reduces the deubiquitination of HDGF-p62 signaling to stimulate autophagy and induce primary cisplatin chemosensitivity in nasopharyngeal carcinoma [ Mol Cancer, 2025, 24(1):152] PubMed: 40414865
The adaptor protein AP-3β disassembles heat-induced stress granules via 19S regulatory particle in Arabidopsis [ Nat Commun, 2025, 16(1):2039] PubMed: 40016204
ACSS2 drives senescence-associated secretory phenotype by limiting purine biosynthesis through PAICS acetylation [ Nat Commun, 2025, 16(1):2071] PubMed: 40021646
Cholangiocarcinoma PDHA1 succinylation suppresses macrophage antigen presentation via alpha-ketoglutaric acid accumulation [ Nat Commun, 2025, 16(1):3177] PubMed: 40180922
F-box protein FBXO32 ubiquitinates and stabilizes D-type cyclins to drive cancer progression [ Nat Commun, 2025, 16(1):4060] PubMed: 40307251
Engineered Spirulina platensis for treating rheumatoid arthritis and restoring bone homeostasis [ Nat Commun, 2025, 16(1):4434] PubMed: 40360534

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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