D-Galactose

製品コードS3849 バッチS384902

化学情報

	Synonyms	D-Galactopyranose, D-(+)-Galactose	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₆H₁₂O₆
	分子量	180.16	CAS No.	59-23-4
Solubility (25°C)*	体外	DMSO	36 mg/mL (199.82 mM)
		Water	36 mg/mL (199.82 mM)
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	D-Galactose (D-Galactopyranose, D-(+)-Galactose) is an aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins and is converted enzymatically into D-glucose for metabolism or polysaccharides for storage. It accelerates senescence in invertebrates and mammals and has been used as a model for aging.
in vitro	D-Galactose (D-gal) can induce oxidative stress in non-cancer cells and result in cell damage by disturbing glucose metabolism. D-Gal treatment can transcriptionally up-regulate the genes relevant to necroptosis (Bmf, Bnip3) and autophagy (Atg5, TIGAR) but not the genes related to apoptosis (Caspase3, Bax, and p53). D-Gal does not activate Caspase-3, but prompts puncta-like GFP–LC3 distribution, an indicator for activated autophagy^[1].

製品説明

D-Galactose (D-Galactopyranose, D-(+)-Galactose) is an aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins and is converted enzymatically into D-glucose for metabolism or polysaccharides for storage. It accelerates senescence in invertebrates and mammals and has been used as a model for aging.

in vitro

D-Galactose (D-gal) can induce oxidative stress in non-cancer cells and result in cell damage by disturbing glucose metabolism. D-Gal treatment can transcriptionally up-regulate the genes relevant to necroptosis (Bmf, Bnip3) and autophagy (Atg5, TIGAR) but not the genes related to apoptosis (Caspase3, Bax, and p53). D-Gal does not activate Caspase-3, but prompts puncta-like GFP–LC3 distribution, an indicator for activated autophagy^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Mouse neuroblastoma cell line Neuro2a (N2a), human neuroblastoma cell line SH-SY5Y, human prostate cancer cell line PC-3 and mouse fibroblast cell line NIH3T3; Human hepatoma cell line HepG2 and human hepatocyte cell line LO2
	濃度	0-60 g/L
	反応時間	24 h
	実験の流れ	Mouse neuroblastoma cell line Neuro2a (N2a), human neuroblastoma cell line SH-SY5Y, human prostate cancer cell line PC-3 and mouse fibroblast cell line NIH3T3 are cultured in DMEM supplemented with 10% FBS, 100 U/ml penicillin, and 100 mg/ml streptomycin. Human hepatoma cell line HepG2 and human hepatocyte cell line LO2 are cultured in RPMI 1640 with 10% FBS. Cells are maintained at 37℃ with 5% CO2, and treated with D-gal or glucose in the concentrations and the time periods. Morphological assessments are conducted under microscope. Cell viability is evaluated by MTT assay.

参考

[1] Li N, et al. J Cell Biochem. 2011, 112(12):3834-44.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Iron derived from NCOA4-mediated ferritinophagy causes cellular senescence via the cGAS-STING pathway [ Cell Death Discov, 2023, 10.1038/s41420-023-01712-7]	PubMed: 37980349
Hypoxia-dependent expression of MAP17 coordinates the Warburg effect to tumor growth in hepatocellular carcinoma [ J Exp Clin Cancer Res, 2021, 40(1):121]	PubMed: 33832535
Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine. [ J Exp Med, 2020, 217(2)]	PubMed: 31753849

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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