D-Galactose

製品コードS3849 バッチS384902

印刷

化学情報

Synonyms

D-Galactopyranose, D-(+)-Galactose

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₆H₁₂O₆

分子量

180.16

CAS No.

59-23-4

Solubility (25°C)*

体外

DMSO

36 mg/mL (199.82 mM)

Water

36 mg/mL (199.82 mM)

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	D-Galactose (D-Galactopyranose, D-(+)-Galactose) is an aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins and is converted enzymatically into D-glucose for metabolism or polysaccharides for storage. It accelerates senescence in invertebrates and mammals and has been used as a model for aging.D-Galactose can be used to induce animal models of Senescence.
in vitro	D-Galactose (D-gal) can induce oxidative stress in non-cancer cells and result in cell damage by disturbing glucose metabolism. This compound can transcriptionally up-regulate the genes relevant to necroptosis (Bmf, Bnip3) and autophagy (Atg5, TIGAR) but not the genes related to apoptosis (Caspase3, Bax, and p53). It does not activate Caspase-3, but prompts puncta-like GFP–LC3 distribution, an indicator for activated autophagy^[1].

製品説明

D-Galactose (D-Galactopyranose, D-(+)-Galactose) is an aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins and is converted enzymatically into D-glucose for metabolism or polysaccharides for storage. It accelerates senescence in invertebrates and mammals and has been used as a model for aging.D-Galactose can be used to induce animal models of Senescence.

in vitro

D-Galactose (D-gal) can induce oxidative stress in non-cancer cells and result in cell damage by disturbing glucose metabolism. This compound can transcriptionally up-regulate the genes relevant to necroptosis (Bmf, Bnip3) and autophagy (Atg5, TIGAR) but not the genes related to apoptosis (Caspase3, Bax, and p53). It does not activate Caspase-3, but prompts puncta-like GFP–LC3 distribution, an indicator for activated autophagy^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Mouse neuroblastoma cell line Neuro2a (N2a), human neuroblastoma cell line SH-SY5Y, human prostate cancer cell line PC-3 and mouse fibroblast cell line NIH3T3; Human hepatoma cell line HepG2 and human hepatocyte cell line LO2
	濃度	0-60 g/L
	反応時間	24 h
	実験の流れ	Mouse neuroblastoma cell line Neuro2a (N2a), human neuroblastoma cell line SH-SY5Y, human prostate cancer cell line PC-3 and mouse fibroblast cell line NIH3T3 are cultured in DMEM supplemented with 10% FBS, 100 U/ml penicillin, and 100 mg/ml streptomycin. Human hepatoma cell line HepG2 and human hepatocyte cell line LO2 are cultured in RPMI 1640 with 10% FBS. Cells are maintained at 37℃ with 5% CO2, and treated with D-gal or glucose in the concentrations and the time periods. Morphological assessments are conducted under microscope. Cell viability is evaluated by MTT assay.

参考

https://pubmed.ncbi.nlm.nih.gov/21826710/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Downregulated TFPI2 Accelerates Skin Aging by Repressing the Cell Cycle through Phosphoinositide 3-Kinase/Protein Kinase B/CDC6 Pathway [ J Invest Dermatol, 2025, S0022-202X(25)00033-8]	PubMed: 39848565
BRD4 plays an antiaging role in the senescence of renal tubular epithelial cells [ Transl Androl Urol, 2024, 13(6):1014-1023]	PubMed: 38983468
Iron derived from NCOA4-mediated ferritinophagy causes cellular senescence via the cGAS-STING pathway [ Cell Death Discov, 2023, 10.1038/s41420-023-01712-7]	PubMed: 37980349
Hypoxia-dependent expression of MAP17 coordinates the Warburg effect to tumor growth in hepatocellular carcinoma [ J Exp Clin Cancer Res, 2021, 40(1):121]	PubMed: 33832535
CD73 is a hypoxia-responsive gene and promotes the Warburg effect of human gastric cancer cells dependent on its enzyme activity [ J Cancer, 2021, 12(21):6372-6382]	PubMed: 34659527
Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine. [ J Exp Med, 2020, 217(2)]	PubMed: 31753849

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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