D-Luciferin

製品コードS7763 バッチS776303

印刷

化学情報

Chemical Structure Synonyms Firefly luciferin, Beetle Luciferin Storage
(From the date of receipt)		 3 years -20°C(in the dark) powder
化学式

C11H8N2O3S2
分子量 280.32 CAS No. 2591-17-5
Solubility (25°C)* 体外 DMSO 56 mg/mL (199.77 mM)
Ethanol 1 mg/mL (3.56 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 D-Luciferin is a popular bioluminescent substrate of luciferase in the presence of ATP, used in luciferase-based bioluminescence imaging and cell-based high-throughput screening applications. In an immunocompetent mouse model of ovarian cancer, the use of D-luciferin substrate and firefly luciferase preserves tumour-host immune interactions since bioluminescent imaging is a more sensitive indication of tumour growth than weight gain.
in vitro

1.Example protocol for in vitro bioluminescent image assays
a) Prepare a 100 mM (100-200X) Luciferin stock solution in sterile water. Mix well. Use immediately, or make single use aliquots, and store at -20℃, avoid freeze-thaw cycles, avoid exposure to the light.
b) Prepare a 0.5-1 mM working solution of D-Luciferin in pre-warmed tissue culture medium.
c) Aspirate media from cultured cells.
d) Add this compound working solution to cells, and incubate the cells for 5-10 minutes at 37℃ just prior to imaging.
2.Example protocol for in vivo bioluminescent image assays
a) Prepare a 15 mg/mL Luciferin stock solution in DPBS, without Mg2+ and Ca2+. Mix well.
b) Filter sterilizes the solution through a 0.2 μm filter. Use immediately, or make single use aliquots, and store at -20℃, avoid freeze-thaw cycles, avoid exposure to the light.
c) Inject the luciferin intra-peritoneally (i.p.) 10-15 minutes before imaging at 150 mg/kg (or 10 μL/g of this chemical stock solution) of the animal body weight.
Note: A kinetic study of luciferin should be performed for each animal model to determine peak signal time.
3.Example protocol for luciferin reporter assays
a) Prepare a 100 mM Luciferin stock solution in sterile water. Use immediately, or make single use aliquots, and store at -20℃, avoid freeze-thaw cycles, avoid exposure to the light.
b) Prepare a 1 mM working solution of this compound with 3 mM ATP, 1 mM DTT and 15 mM MgSO4 in 25 mM tricine buffer pH 7.8.
c) Pipette 5-10 μLof cell lysate into a microplate. Use lysis reagent or buffer without lysate as a blank.
d) Prime luminometer with this chemical working solution.
e) Inject 200 μL of this compound working solution with no delay and a 10 second integration time.
in vivo

In an immunocompetent mouse model of ovarian cancer, use of D-luciferin substrate and firefly luciferase preserves tumor-host immune interactions, since bioluminescent imaging is a more sensitive indication of tumor growth than weight gain. [2]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 ID8 cells
濃度 3000 μg/ml
反応時間
実験の流れ
動物実験 動物モデル Female BALB/c nu/nu mice
投薬量 75 mg/kg
投与方法 s.c.

参考

  • https://pubmed.ncbi.nlm.nih.gov/3694139/
  • https://pubmed.ncbi.nlm.nih.gov/25992288/
  • https://pubmed.ncbi.nlm.nih.gov/20671955/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Immunogenic dying cells elicit potent anti-tumor T cell immunity against lung metastasis and tumorigenesis [ J Cancer Res Clin Oncol, 2025, 151(1):38] PubMed: 39825073
Gut-derived metabolite 3-methylxanthine enhances cisplatin-induced apoptosis via dopamine receptor D1 in a mouse model of ovarian cancer [ mSystems, 2024, 9(7):e0130123] PubMed: 38899930
Carbenoxolone disodium suppresses the migration of gastric cancer by targeting HDAC6 [ Future Med Chem, 2023, 15(4):333-344] PubMed: 36946221
Crosstalk between pro-survival sphingolipid metabolism and complement signaling induces inflammasome-mediated tumor metastasis [ Cell Rep, 2022, 41(10):111742] PubMed: 36476873
A magneto-controlled biocatalytic cascade with a fluorescent output [ Org Biomol Chem, 2022, 20(9):1869-1873] PubMed: 35156979
Dysregulated Glutamate Transporter SLC1A1 Propels Cystine Uptake via Xc- for Glutathione Synthesis in Lung Cancer [ Cancer Res, 2021, 81(3):552-566] PubMed: 33229341
Leronlimab, a humanized monoclonal antibody to CCR5, blocks breast cancer cellular metastasis and enhances cell death induced by DNA damaging chemotherapy [ Breast Cancer Res, 2021, 23(1):11] PubMed: 33485378
KDM4A-mediated histone demethylation of SLC7A11 inhibits cell ferroptosis in osteosarcoma [ Biochem Biophys Res Commun, 2021, 550:77-83] PubMed: 33689883
Dysregulated Glutamate Transporter SLC1A1 Propels Cystine Uptake via Xc- for Glutathione Synthesis in Lung Cancer [ Cancer Res, 2020, canres.0617.2020] PubMed: 33229341
Tumor treating fields increases membrane permeability in glioblastoma cells [ Cell Death Discov, 2018, 4:113] PubMed: 30534421

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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