Dac51

製品コードS9876 バッチS987602

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₂₀H₁₇Cl₂F₃N₄O₄

分子量

505.27

CAS No.

2243944-92-3 (free base)

Solubility (25°C)*

体外

DMSO

100 mg/mL (197.91 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	5.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 Corn oil	5.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Dac51 is a potent FTO inhibitor with an IC50 of 0.4 μM for inhibiting FTO demethylation.
in vitro	Dac51 can serve as a potent FTO inhibitor to dampen the glycolytic capacity of tumor cells by inhibiting FTO-mediated demethylation on transcripts including Jun and Cebpb.[1]
in vivo	Dac51, a FTO inhibitor, delivers strong T cell-mediated antitumor effects and prevents tumor recurrence via increasesing CD8+ T cell inﬁltration in tumors, in multiple vivo cancer models.[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	B16-OVA cells, LLC cell line, LLC-zsGreen-OTIp cells, MC38 cell line
	濃度	1 μM, 5 μM
	反応時間	48 h
	実験の流れ	Cells are cultured in Dulbecco s modified Eagles medium supplemented with 10% heat-inactivated fetal bovine serum, 2 mM L-glutamine, 10 mM HEPES, and 1% non-essential amino acids at 37 ℃ 5% CO2. Cells are cultured with Dac51 (1 mM, 5mM) for 48 hr.Then cell growth is monitored by Trypan Blue staining. Apoptosis is analyzed through ﬂow cytometry. Cell total RNA is extracted for m6A dot blot assay.
動物実験	動物モデル	C57BL/6 mice inoculated with Fto-Kd or B16-OVA tumor cells, Rag2/ mice inoculated with B16-OVA cells
	投薬量	2 mg/kg
	投与方法	i.p.

参考

[1] Yi Liu, et al. Cell Metab. 2021 Jun 1;33(6):1221-1233.e11.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

The Functional Role and Regulatory Mechanism of FTO m6A RNA Demethylase in Human Uterine Leiomyosarcoma [ Int J Mol Sci, 2023, 24(9)7957]	PubMed: 37175660

The Functional Role and Regulatory Mechanism of FTO m6A RNA Demethylase in Human Uterine Leiomyosarcoma [ Int J Mol Sci, 2023, 24(9)7957]

PubMed: 37175660

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。