Danofloxacin Mesylate

製品コードS3058 バッチS305801

印刷

化学情報

Chemical Structure Synonyms CP-76136-27 Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C19H20FN3O3.CH4O3S
分子量 453.48 CAS No. 119478-55-6
Solubility (25°C)* 体外 Water 91 mg/mL (200.67 mM)
DMSO Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
Saline
30.0mg/ml Taking the 1 mL working solution as an example, add 30 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Danofloxacin (CP-76136-27) is a fluoroquinolone antibacterial for veterinary use, with MIC90 of 0.28 μM.
in vitro Danofloxacin is a synthetic antibacterial agent of the fluoroquinolone class, acts principally by the inhibition of bacterial DNA-gyrase, which is necessary for supercoiling of DNA to provide a suitable spatial arrangement of DNA within the bacterial cell. The minimum inhibitory concentration of danofloxacin against 90% (MIC90) of contemporary European and North American field isolates of Pasteurella haemolytica, Pasteurella multocida and Haemophilus somnus, the most important bacterial respiratory pathogens of cattle, is 0.125 μg/ml. [1]
in vivo Danofloxacin shows protective dose (PD50) of 0.38, 0.8, 2.42 mg/kg for P. multocida, E. coli and S. choleraesuis in in vivo mouse protection assay. [2]

プロトコル(参考用のみ)

キナーゼアッセイ Minimum inhibitory concentration
The bacteria examined are isolated from clinical cases of respiratory disease in cattle from North America and Europe. The minimum inhibitory concentration (MIC) of danofloxacin against these isolates is determined using the Sensititre susceptibility testing system, a micro-adaptation of the broth dilution method. Following overnight incubation, isolated pure colonies of the test bacterium, grown on non-inhibitory media, are emulsified in 4 ml of sterile distilled water. The cell suspension is homogenized by vortex mixing and the density adjusted to 0.5 on the MacFarland Standard. Ten microlitres of this suspension are then aseptically transferred to cationsupplemented Mueller-Hinton broth with 1.14% TES buffer. The inoculated broth is homogenized by vortex mixing. Fifty microlitres of the resulting cell suspension are dispensed into each well of a pre-dosed Sensititre plate. Inoculated plates are covered with adhesive cellophane, incubated at 37癈 for 18 h, and then read using an inverted mirror reader. The minimum inhibitory concentration is defined as the lowest concentration of an antibacterial that inhibited visible growth.
動物実験 動物モデル mice
投薬量 0-10 mg/kg
投与方法 p. o or s. c.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。