Datasheet

生物学的記述

Specificity	DEC1 Antibody [D7J6] detects endogenous levels of total DEC1 protein.
Background	DEC1 (BHLHE40) belongs to the basic helix-loop-helix family of transcription factors alongside DEC2, characterized by a DNA-binding basic domain and helix-loop-helix dimerization motif that recognizes E-box sequences (CACGTG) in target gene promoters to modulate circadian rhythm, differentiation, and stress responses. DEC1 organizes an N-terminal transactivation domain, central Orange domain for heterodimer selectivity, and nuclear localization signals flanking the bHLH region that confers specificity for class C E-boxes while repressing CLOCK-BMAL1 heterodimers essential for circadian oscillation. DEC1 transcriptionally represses pro-apoptotic genes under serum deprivation by stabilizing cyclin E and inhibiting caspase activation through selective suppression of mitochondrial pathways, while hypoxia-inducible factor signaling induces DEC1 expression that cooperates with HIF1α to transactivate VEGF and glycolytic enzymes for adaptive metabolic reprogramming. Interaction with p53 modulates DNA damage responses, where DEC1 attenuates MIC-1 induction but enhances survivin to balance survival versus death, alongside direct binding to TGF-β1 promoters that amplifies Smad3 phosphorylation and SNAIL expression driving epithelial-mesenchymal transition through N-cadherin upregulation and E-cadherin repression. DEC1 coordinates chondrocyte maturation and osteoblast differentiation during skeletal development, with rhythmic expression in the suprachiasmatic nucleus maintaining peripheral clock entrainment.

Specificity

DEC1 Antibody [D7J6] detects endogenous levels of total DEC1 protein.

Background

DEC1 (BHLHE40) belongs to the basic helix-loop-helix family of transcription factors alongside DEC2, characterized by a DNA-binding basic domain and helix-loop-helix dimerization motif that recognizes E-box sequences (CACGTG) in target gene promoters to modulate circadian rhythm, differentiation, and stress responses. DEC1 organizes an N-terminal transactivation domain, central Orange domain for heterodimer selectivity, and nuclear localization signals flanking the bHLH region that confers specificity for class C E-boxes while repressing CLOCK-BMAL1 heterodimers essential for circadian oscillation. DEC1 transcriptionally represses pro-apoptotic genes under serum deprivation by stabilizing cyclin E and inhibiting caspase activation through selective suppression of mitochondrial pathways, while hypoxia-inducible factor signaling induces DEC1 expression that cooperates with HIF1α to transactivate VEGF and glycolytic enzymes for adaptive metabolic reprogramming. Interaction with p53 modulates DNA damage responses, where DEC1 attenuates MIC-1 induction but enhances survivin to balance survival versus death, alongside direct binding to TGF-β1 promoters that amplifies Smad3 phosphorylation and SNAIL expression driving epithelial-mesenchymal transition through N-cadherin upregulation and E-cadherin repression. DEC1 coordinates chondrocyte maturation and osteoblast differentiation during skeletal development, with rhythmic expression in the suprachiasmatic nucleus maintaining peripheral clock entrainment.

使用情報

Application

WB, IP, ELISA

Dilution

WB	IP
1:100-1:1000	1:100-1:200

Reactivity

Human, Mouse, Rat

Source

Mouse Monoclonal Antibody

MW

43 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/26819638/
https://pubmed.ncbi.nlm.nih.gov/12119049/

Application Data

WB

Validated by Selleck

Lane 1: HepG2