Demethylzeylasteral (T-96)

製品コードS3608 バッチS360802

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C29H36O6
分子量 480.59 CAS No. 107316-88-1
Solubility (25°C)* 体外 DMSO 96 mg/mL (199.75 mM)
Ethanol 16 mg/mL (33.29 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Demethylzeylasteral (T-96), the active component isolated from Tripterygium wilfordii Hook F., inhibits UDP-glucuronosyltransferase (UGT) isoforms UGT1A6 and UGT2B7 with immunosuppressive effects.
in vitro Demethylzeylasteral shows strong inhibition towards UGT1A6 and UGT2B7, with negligible influence towards UGT1A9[1]. Demethylzeylasteral has an anti-tumor property in melanoma cells. Demethylzeylasteral not only inhibits cell proliferation through cell cycle arrest at S phase, but also induces cell apoptosis in melanoma cells. CDK2 and Cycin E1 decrease in a dose-dependent manner after Demethylzeylasteral treatment. Demethylzeylasteral inhibits MCL1, whose overexpression recovers the proliferation ability inhibited by demethylzeylasteral. Demethylzeylasteral inhibits clonogenicity and tumorigenesis in melanoma cells through downregulating the expression of MCL1. Demethylzeylasteral is found to inhibit Ca2+ currents in mouse spermatogonia and has an antifertility ability[2].
in vivo T-96 demonstrates a significant proteinuria reduction both in a time and concentration-dependent manner. T-96 significantly inhibits the activation of NF-kB in the kidneys of MRL/lpr mice. In addition, T-96 reduces the secretion of pro-inflammatory mediators such as TNF-α, COX-2 and ICAM-1[3].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 melanoma cell lines, MV3 and A375
濃度 1, 5, 10 and 20 μM
反応時間 48 h
実験の流れ

Melanoma cell lines, MV3 and A375, are treated with different concentrations of demethylzeylasteral (1, 5, 10 and 20 μM, dimethyl sulfoxide (DMSO) is used as control) for 48 h.
動物実験 動物モデル C57BL/6 mice
投薬量 1.2 and 0.6 mg/10g
投与方法 by gavage

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Demethylzelasteral inhibits proliferation and EMT via repressing Wnt/β-catenin signaling in esophageal squamous cell carcinoma [ J Cancer, 2021, 12(13):3967-3975] PubMed: 34093803
T-96 attenuates inflammation by inhibiting NF-κB in adjuvant-induced arthritis. [ Front Biosci (Landmark Ed), 2020, 25:498-512] PubMed: 31585899

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。