Dimercaprol

化学情報

生物活性

プロトコル(参考用のみ)

参考

製品コードS4446 バッチS444601

	Synonyms	Bal, 2,3-Dimercapto-1-propanol, Dithioglycerol	Storage (From the date of receipt)	2 years -20°C liquid
	化学式	C₃H₈OS₂
	分子量	124.22	CAS No.	59-52-9
Solubility (25°C)*	体外


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

細胞アッセイ	細胞株	PC-12 cells
	濃度	10, 25, 50 and 100 μM
	反応時間	4 h
	実験の流れ	Cell viability was tested by WST-1 ([2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium]) assay. Both dimercaprol and acrolein were dissolved in Hank’s Balanced Salt Solution (HBSS). The final concentration of acrolein was 100 μM. The final concentrations of dimercaprol were 10, 25, 50 and 100 μM. PC-12 cells were seeded in 12-well plates at the concentration of 10⁵ cells/well. After overnight incubation, acrolein was added into each well of acrolein group and treatment groups. Equal volumes of HBSS were added into wells of the negative control group. After incubation for 15 minutes, dimercaprol was added at the desired final concentrations (10, 25, 50 and 100 μM) into wells of treatment groups and equal volume of HBSS was added into other wells. The initial 15-minute incubation in acrolein was designed to mimic pathological conditions by allowing acrolein to enter the cells. WST-1 assay solution was added into every well after incubating 4 h, and then allowed to incubate for an additional 2 h.
動物実験	動物モデル	Male Sprague–Dawley rats of spinal cord contusion injury model
	投薬量	5 mg/kg
	投与方法	i.p

Dimercaprol (Bal、2,3-ジメルカプト-1-プロパノール、ジチオグリセロール) は、インビトロでHIV-1 tat活性、ウイルス産生、および感染性を阻害するアクロレイン捕捉剤です。

in vitro

Dimercaprol exerts its acrolein scavenging capability, significantly protects PC-12 cells from acrolein-mediated cell death in a dose dependent manner.

Dimercaprol could significantly reduce acrolein contents in spinal cord tissue following a spinal cord contusion injury in rats.

https://pubmed.ncbi.nlm.nih.gov/28301040/
https://pubmed.ncbi.nlm.nih.gov/2202353/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429218/

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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