DPI (Diphenyleneiodonium chloride)

製品コードS8639 バッチS863901

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₂H₈I.Cl

分子量

314.55

CAS No.

4673-26-1

Solubility (25°C)*

体外

DMSO

13 mg/mL (41.32 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	DPI (Diphenyleneiodonium chloride) is an inhibitor of NADPH oxidase and also a potent, irreversible, and time-, temperature-dependent iNOS/eNOS inhibitor. Diphenyleneiodonium chloride (DPI) also functions as a TRPA1 activator and selectively inhibits intracellular reactive oxygen species (ROS).
in vitro	DPI (Diphenyleneiodonium chloride) inhibits the activity of NADPH oxidase, nitric oxide synthase, xanthine oxidase and NADPH cytochrome P450 oxidoreductase^[4]. Femtomolar concentrations of this compound exert potent anti-inflammatory and neuroprotective effects by inhibiting microglial activation through the inhibition of ERK-regulated PHOX activity^[1]. It has frequently been used to inhibit ROS production mediated by various flavoenzymes, including NAD(P)H oxidase, quinone oxidoreductase, cytochrome P450 reductase and nitric oxide synthase^[2]. NADPH, NADP+, and 2'5'-ADP blocks the inhibitory action of DPI^[3]. Treatment with this compound in ARPE-19 cells evoked a dose- and time-dependent growth inhibition, and also induced DNA fragmentation and protein content of the proapoptotic factor Bax. In addition, it signiﬁcantly induced the expression and phosphorylation of p53, which induces proapoptotic genes in response to DNA damage or irreparable cell cycle arrest. ROS have been implicated as a key factor in the activation of p53 by many chemotherapeutic drugs^[4].
in vivo	Diphenyleneiodonium (DPI), an NADPH oxidase inhibitor, inhibited the production of pro-inflammatory cytokines, (TNF-α and IL-6), reduced macrophage infiltration and classical polarization, and induced the ROS generation.

製品説明

DPI (Diphenyleneiodonium chloride) is an inhibitor of NADPH oxidase and also a potent, irreversible, and time-, temperature-dependent iNOS/eNOS inhibitor. Diphenyleneiodonium chloride (DPI) also functions as a TRPA1 activator and selectively inhibits intracellular reactive oxygen species (ROS).

in vitro

DPI (Diphenyleneiodonium chloride) inhibits the activity of NADPH oxidase, nitric oxide synthase, xanthine oxidase and NADPH cytochrome P450 oxidoreductase^[4].

Femtomolar concentrations of this compound exert potent anti-inflammatory and neuroprotective effects by inhibiting microglial activation through the inhibition of ERK-regulated PHOX activity^[1].

It has frequently been used to inhibit ROS production mediated by various flavoenzymes, including NAD(P)H oxidase, quinone oxidoreductase, cytochrome P450 reductase and nitric oxide synthase^[2].

NADPH, NADP+, and 2'5'-ADP blocks the inhibitory action of DPI^[3].

Treatment with this compound in ARPE-19 cells evoked a dose- and time-dependent growth inhibition, and also induced DNA fragmentation and protein content of the proapoptotic factor Bax. In addition, it signiﬁcantly induced the expression and phosphorylation of p53, which induces proapoptotic genes in response to DNA damage or irreparable cell cycle arrest. ROS have been implicated as a key factor in the activation of p53 by many chemotherapeutic drugs^[4].

in vivo

Diphenyleneiodonium (DPI), an NADPH oxidase inhibitor, inhibited the production of pro-inflammatory cytokines, (TNF-α and IL-6), reduced macrophage infiltration and classical polarization, and induced the ROS generation.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	ARPE-19 cells
	濃度	0.1, 1, and 10 μM
	反応時間	6, 12, 24, and 48 h
	実験の流れ	ARPE-19 cells are plated at 1×10⁶ cells per 60-mm dishes and incubated for 24 h. Cells are cultured in presence or absence of different concentrations of DPI (Diphenyleneiodonium chloride) in fresh DMEM/F12 medium supplemented with 10% FBS. After incubation, the cells are trypsinized, washed with phosphate-buffered saline (PBS) and the viable cells were scored by the trypan blue dye exclusion method using a hemocytometer.
動物実験	動物モデル	Male C57BL/6 mice
	投薬量	1 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/21804230/
https://pubmed.ncbi.nlm.nih.gov/9878531/
https://pubmed.ncbi.nlm.nih.gov/1703974/

https://pubmed.ncbi.nlm.nih.gov/17184774/
https://pubmed.ncbi.nlm.nih.gov/32550901/

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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