DPI (Diphenyleneiodonium chloride)

製品コードS8639 バッチS863902

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₂H₈I.Cl

分子量

314.55

CAS No.

4673-26-1

Solubility (25°C)*

体外

DMSO

18 mg/mL (57.22 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	3%DMSO 1 40%PEG300 2 2%Tween80 3 55%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.500mg/ml (1.59mM)	Taking the 1 mL working solution as an example, add 30 μL of clarified DMSO stock solution of 16.6666666666667 mg/ml to 400 μL of PEG300, mix evenly to clarify it; add 20 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 550 μL of ddH2O to make it clear. Volume up to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	DPI (Diphenyleneiodonium chloride) はNADPH oxidase阻害剤であり、強力で不可逆的な時間・温度依存的なiNOS/eNOS阻害剤でもあります。Diphenyleneiodonium chloride (DPI) はTRPA1活性化剤としても機能し、細胞内reactive oxygen species (ROS)を選択的に阻害します。
in vitro	Diphenyleneiodonium chloride (DPI) inhibits the activity of NADPH oxidase, nitric oxide synthase, xanthine oxidase and NADPH cytochrome P450 oxidoreductase. Femtomolar concentrations of this compound exert potent anti-inflammatory and neuroprotective effects by inhibiting microglial activation through the inhibition of ERK-regulated PHOX activity. It has frequently been used to inhibit ROS production mediated by various flavoenzymes, including NAD(P)H oxidase, quinone oxidoreductase, cytochrome P450 reductase and nitric oxide synthase. NADPH, NADP+, and 2'5'-ADP blocks its inhibitory action. Treatment with DPI in ARPE-19 cells evoked a dose- and time-dependent growth inhibition, and also induced DNA fragmentation and protein content of the proapoptotic factor Bax. In addition, it signiﬁcantly induced the expression and phosphorylation of p53, which induces proapoptotic genes in response to DNA damage or irreparable cell cycle arrest. ROS have been implicated as a key factor in the activation of p53 by many chemotherapeutic drugs.
in vivo	Diphenyleneiodonium chloride (DPI), an NADPH oxidase inhibitor, inhibited the production of pro-inflammatory cytokines (TNF-α and IL-6), reduced macrophage infiltration and classical polarization, and induced ROS generation.

製品説明

DPI (Diphenyleneiodonium chloride) はNADPH oxidase阻害剤であり、強力で不可逆的な時間・温度依存的なiNOS/eNOS阻害剤でもあります。Diphenyleneiodonium chloride (DPI) はTRPA1活性化剤としても機能し、細胞内reactive oxygen species (ROS)を選択的に阻害します。

in vitro

Diphenyleneiodonium chloride (DPI) inhibits the activity of NADPH oxidase, nitric oxide synthase, xanthine oxidase and NADPH cytochrome P450 oxidoreductase.

Femtomolar concentrations of this compound exert potent anti-inflammatory and neuroprotective effects by inhibiting microglial activation through the inhibition of ERK-regulated PHOX activity.

It has frequently been used to inhibit ROS production mediated by various flavoenzymes, including NAD(P)H oxidase, quinone oxidoreductase, cytochrome P450 reductase and nitric oxide synthase.

NADPH, NADP+, and 2'5'-ADP blocks its inhibitory action.

Treatment with DPI in ARPE-19 cells evoked a dose- and time-dependent growth inhibition, and also induced DNA fragmentation and protein content of the proapoptotic factor Bax. In addition, it signiﬁcantly induced the expression and phosphorylation of p53, which induces proapoptotic genes in response to DNA damage or irreparable cell cycle arrest. ROS have been implicated as a key factor in the activation of p53 by many chemotherapeutic drugs.

in vivo

Diphenyleneiodonium chloride (DPI), an NADPH oxidase inhibitor, inhibited the production of pro-inflammatory cytokines (TNF-α and IL-6), reduced macrophage infiltration and classical polarization, and induced ROS generation.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	ARPE-19 cells
	濃度	0.1, 1, and 10 μM
	反応時間	6, 12, 24, and 48 h
	実験の流れ	ARPE-19 cells are plated at 1×10⁶ cells per 60-mm dishes and incubated for 24 h. After incubation with different concentrations of DPI (Diphenyleneiodonium chloride) in fresh DMEM/F12 medium supplemented with 10% FBS, either in its presence or absence, the cells are trypsinized, washed with phosphate-buffered saline (PBS) and the viable cells were scored by the trypan blue dye exclusion method using a hemocytometer.
動物実験	動物モデル	Male C57BL/6 mice
	投薬量	1 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/21804230/
https://pubmed.ncbi.nlm.nih.gov/9878531/
https://pubmed.ncbi.nlm.nih.gov/1703974/

https://pubmed.ncbi.nlm.nih.gov/17184774/
https://pubmed.ncbi.nlm.nih.gov/32550901/

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人間や獣医の診断であるか治療的な使用のためにでない。

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