DMOG

製品コードS7483 バッチS748303

印刷

化学情報

Chemical Structure Synonyms Dimethyloxalylglycine Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C6H9NO5
分子量 175.14 CAS No. 89464-63-1
Solubility (25°C)* 体外 DMSO 35 mg/mL (199.84 mM)
Water 35 mg/mL (199.84 mM)
Ethanol 35 mg/mL (199.84 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 DMOG (Dimethyloxalylglycine) is an antagonist of α-ketoglutarate cofactor and inhibitor for HIF prolylhydroxylase, leading to stabilisation and accumulation of HIF-1α protein in the nucleus. DMOG enhances autophagy.
in vitro

DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. [1] DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC. [3]
in vivo

DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice. [2] Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats. [4]
密度 1.246 g/mL

プロトコル(参考用のみ)

細胞アッセイ 細胞株 VSMC cells
濃度 ~100 μM
反応時間 72-96 hours
実験の流れ

To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.
動物実験 動物モデル Mice (C57Bl6)
投薬量 8 mg in 0.5 ml saline
投与方法 i.p.

カスタマーフィードバック

Data from [Data independently produced by , , Hepatology, 2018, 68(6):2359-2375]

Data from [Data independently produced by , , Theranostics, 2017, 7(13): 3293-3305]

Data from [Data independently produced by , , Theranostics, 2017, 7(13):3293-3305]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Hypoxia stabilizes SETDB1 to maintain genome stability [ Nucleic Acids Res, 2023, gkad796] PubMed: 37850636
An inducible long noncoding RNA, LncZFHX2, facilitates DNA repair to mediate osteoarthritis pathology [ Redox Biol, 2023, 66:102858] PubMed: 37633048
A mitochondrial EglN1-AMPKα axis drives breast cancer progression by enhancing metabolic adaptation to hypoxic stress [ EMBO J, 2023, 42(20):e113743] PubMed: 37661833
An inducible long noncoding RNA, LncZFHX2, facilitates DNA repair to mediate osteoarthritis pathology [ Redox Biol, 2023, 66:102858] PubMed: 37633048
Stellate ganglion block ameliorated central post-stroke pain with comorbid anxiety and depression through inhibiting HIF-1α/NLRP3 signaling following thalamic hemorrhagic stroke [ J Neuroinflammation, 2023, 20(1):82] PubMed: 36944982
Hypoxia-inducible factor 1 signaling drives placental aging and can provoke preterm labor [ Elife, 2023, 12RP85597] PubMed: 37610425
Hypoxia Primes Human ISCs for Interleukin-Dependent Rescue of Stem Cell Activity [ Cell Mol Gastroenterol Hepatol, 2023, 10.1016/j.jcmgh.2023.07.012] PubMed: 37562653
Hypoxia Primes Human ISCs for Interleukin-Dependent Rescue of Stem Cell Activity [ Cell Mol Gastroenterol Hepatol, 2023, 16(5):823-846] PubMed: 37562653
An E3 ligase TRIM1 promotes colorectal cancer progression via K63-linked ubiquitination and activation of HIF1α [ Research Square, 2023, 10.21203/rs.3.rs-3493586/v1] PubMed: none
Hypoxia-induced lncRNA STEAP3-AS1 activates Wnt/β-catenin signaling to promote colorectal cancer progression by preventing m6A-mediated degradation of STEAP3 mRNA [ Mol Cancer, 2022, 21(1):168] PubMed: 35986274

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。