Doxycycline

製品コードS5159 バッチS515904

化学情報

	Synonyms	Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₂₂H₂₄N₂O₈
	分子量	444.43	CAS No.	564-25-0
Solubility (25°C)*	体外	DMSO	89 mg/mL (200.25 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Doxycycline（Vibramycin、Doxytetracycline、Doxiciclina、Doxycyclinum）は、細菌や原虫によって引き起こされる多くの種類の感染症の治療に使用される抗生物質です。Doxycyclineは非特異的なマトリックスメタロプロテイナーゼ（MMP）阻害剤でもあります。
in vitro	100 ng/mL-5 µg/mL Doxycycline can significantly alter the metabolic profile of the cell, as well as reduce the proliferative rate, though the effect size depends upon the particular cell line used. This compound arrests cells in G1-S phase of the cell cycle in PANC-1 cells and induces the expression of p53 and its downstream target p21. It down-regulates antiapoptotic genes and induces proapoptotic genes. This chemical also induces apoptosis through a Fas/Fas-ligand dependent pathway in Jurkat T lymphocytes.
in vivo	Doxycycline successfully reduces tumor growth in vivo (inhibited pancreatic cancer cell growth).

製品説明

Doxycycline（Vibramycin、Doxytetracycline、Doxiciclina、Doxycyclinum）は、細菌や原虫によって引き起こされる多くの種類の感染症の治療に使用される抗生物質です。Doxycyclineは非特異的なマトリックスメタロプロテイナーゼ（MMP）阻害剤でもあります。

in vitro

100 ng/mL-5 µg/mL Doxycycline can significantly alter the metabolic profile of the cell, as well as reduce the proliferative rate, though the effect size depends upon the particular cell line used.

This compound arrests cells in G1-S phase of the cell cycle in PANC-1 cells and induces the expression of p53 and its downstream target p21. It down-regulates antiapoptotic genes and induces proapoptotic genes. This chemical also induces apoptosis through a Fas/Fas-ligand dependent pathway in Jurkat T lymphocytes.

in vivo

Doxycycline successfully reduces tumor growth in vivo (inhibited pancreatic cancer cell growth).

プロトコル(参考用のみ)

細胞アッセイ	細胞株	U87 astrocytoma cells, lung cancer cell lines H157 and H1437, LNCaP prostate cancer and HeLa cervical cancer cell lines
	濃度	100 ng/mL, 1 µg/mL
	反応時間	24, 72, and 96 hours
	実験の流れ	Cells are seeded in 6 well plates at 50,000 cells per well and treated with Doxycycline (100 ng/mL, 1 µg/mL, or vehicle control). Cells are counted 24, 72, and 96 hours after plating using a particle counter.
動物実験	動物モデル	Male nu/nu mice
	投薬量	35 mg/pellet
	投与方法	s.c.

参考

https://pubmed.ncbi.nlm.nih.gov/23741339/
https://pubmed.ncbi.nlm.nih.gov/19846942/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

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BMP4 initiates and patterns ventral-caudal structures in zebrafish and human pluripotent stem cell aggregates [ EMBO J, 2026, 45(1):210-242]	PubMed: 41286028
The stress-activated kinase p38 mediates non-canonical activation of Src and tyrosine phosphorylation of the adapter protein TAB1 [ J Biol Chem, 2026, 302(3):111200]	PubMed: 41581886
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NINJ1 regulates plasma membrane fragility under mechanical strain [ Nature, 2025, 10.1038/s41586-025-09222-5]	PubMed: 40490006
IGF2BP1 restricts the induction of human primordial germ cell fate in an m6A-dependent manner [ Cell Stem Cell, 2025, S1934-5909(25)00181-X]	PubMed: 40436019
The cAMP-PKA signaling initiates mitosis by phosphorylating Bora [ Nat Commun, 2025, 16(1):7898]	PubMed: 40849432
RNA-binding proteins DND1 and NANOS3 cooperatively suppress the entry of germ cell lineage [ Nat Commun, 2025, 16(1):4792]	PubMed: 40410171
c-JUN: a chromatin repressor that limits mesoderm differentiation in human pluripotent stem cells [ Nucleic Acids Res, 2025, 53(3)gkaf001]	PubMed: 39876710

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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