Doxycycline Hyclate

製品コードS4163 バッチS416305

印刷

化学情報

Synonyms

WC2031

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₂H₂₄N₂O₈.1/2C₂H₆O.HCl.1/2H₂O

分子量

512.94

CAS No.

24390-14-5

Solubility (25°C)*

体外

DMSO

100 mg/mL (194.95 mM)

Water

100 mg/mL (194.95 mM)

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Doxycycline Hyclate is a member of the tetracycline antibiotics group, and is commonly used to treat a variety of infections. It is also an inhibitor of matrix metallo-proteinases (MMP).
in vitro	Doxycycline hyclate 10% (DOXY) has significant toxicity effects on osteoblast cells compared to the control group with a mean cell viability of 84%. Doxycycline hyclate 10% (DOXY) results in a significantly higher alkaline phosphatase activity (approximately 56%) than the control in osteoblasts. Doxycycline hyclate 10% (DOXY) seems to slightly altered the morphology of the osteoblasts. ^[1]
in vivo	Doxycycline hyclate is an effective treatment for acute E. canis infection in dogs. ^[2] Doxycycline hyclate (5 mg/kg, orally) decreases the collagenase activity in corneas obtained from rabbits. Doxycycline hyclate (5 mg/kg, orally) promotes corneal reepithelialization in the rabbit alkali-burn model, a result, perhaps, of the drug's ability to inhibit excessive collagenase activity. ^[3] Doxycycline hyclate results in 43% of prophylaxis in orally treated mice, in contrast, the sustained-release doxycycline hyclate completely protects mice from infection and resultant pathology. ^[4] Doxycycline hyclate results in bioavailability of 70%, Cmin of 1 mg/L, Cmax of 2.3 mg/L and elimination half-life of 7 hours in calves with immature rumen function. ^[5]

製品説明

Doxycycline Hyclate is a member of the tetracycline antibiotics group, and is commonly used to treat a variety of infections. It is also an inhibitor of matrix metallo-proteinases (MMP).

in vitro

Doxycycline hyclate 10% (DOXY) has significant toxicity effects on osteoblast cells compared to the control group with a mean cell viability of 84%. Doxycycline hyclate 10% (DOXY) results in a significantly higher alkaline phosphatase activity (approximately 56%) than the control in osteoblasts. Doxycycline hyclate 10% (DOXY) seems to slightly altered the morphology of the osteoblasts. ^[1]

in vivo

Doxycycline hyclate is an effective treatment for acute E. canis infection in dogs. ^[2]

Doxycycline hyclate (5 mg/kg, orally) decreases the collagenase activity in corneas obtained from rabbits. Doxycycline hyclate (5 mg/kg, orally) promotes corneal reepithelialization in the rabbit alkali-burn model, a result, perhaps, of the drug's ability to inhibit excessive collagenase activity. ^[3]

Doxycycline hyclate results in 43% of prophylaxis in orally treated mice, in contrast, the sustained-release doxycycline hyclate completely protects mice from infection and resultant pathology. ^[4]

Doxycycline hyclate results in bioavailability of 70%, Cmin of 1 mg/L, Cmax of 2.3 mg/L and elimination half-life of 7 hours in calves with immature rumen function. ^[5]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	MEFs
	濃度	100 ng/ml
	反応時間	12 h
	実験の流れ	Cells were treated with various concentrations of drug.
動物実験	動物モデル	Dutch belted rabbits
	投薬量	1.5 or 5 mg/kg
	投与方法	p.o.

参考

https://pubmed.ncbi.nlm.nih.gov/19485832/
https://pubmed.ncbi.nlm.nih.gov/9527787/
https://pubmed.ncbi.nlm.nih.gov/8306657/

https://pubmed.ncbi.nlm.nih.gov/15215128/
https://pubmed.ncbi.nlm.nih.gov/8498009/
https://pubmed.ncbi.nlm.nih.gov/17166729/
https://pubmed.ncbi.nlm.nih.gov/31204282/

+ 展开

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

The assembly of RAB22A/TMEM33/RTN4 initiates a secretory ER-phagy pathway [ Cell Discov, 2025, 11(1):41]	PubMed: 40301304
The NSP5, ORF6 and NSP13 of SARS-CoV-2 Cooperate to Modulate Inflammatory Cell Death Activation [ Adv Sci (Weinh), 2025, 12(41):e03977]	PubMed: 40810650
TAF1 is required for fetal but not adult hematopoiesis in mice [ Dev Cell, 2025, S1534-5807(25)00405-8]	PubMed: 40675153
KSHV hijacks the antiviral kinase IKKε to initiate lytic replication [ PLoS Pathog, 2025, 21(1):e1012856]	PubMed: 39823515
Manganese is a potent inducer of lysosomal activity that inhibits de novo HBV infection [ PLoS Pathog, 2025, 21(1):e1012800]	PubMed: 39746094
DFFB suppresses interferon to enable cancer persister cell regrowth [ bioRxiv, 2025, 2025.08.15.670603]	PubMed: 40894800
CDK9 recruits HUWE1 to degrade RARα and offers therapeutic opportunities for cutaneous T-cell lymphoma [ Nat Commun, 2024, 15(1):10594]	PubMed: 39632829
BMP suppresses Wnt signaling via the Bcl11b-regulated NuRD complex to maintain intestinal stem cells [ EMBO J, 2024, ]	PubMed: 39433900
Dependence of NPPS creates a targetable vulnerability in RAS-mutant cancers [ Acta Pharmacol Sin, 2024, ]	PubMed: 39506063
Swine RNF5 positively regulates the antiviral activity of IFITM1 by mediating the degradation of ABHD16A [ J Virol, 2024, e0127724.]	PubMed: 39601593

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。