Echinacoside

製品コードS3783 バッチS378302

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₃₅H₄₆O₂₀

分子量

786.73

CAS No.

82854-37-3

Solubility (25°C)*

体外

DMSO

100 mg/mL (127.1 mM)

Ethanol

100 mg/mL (127.1 mM)

Water

13 mg/mL (16.52 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Echinacoside, a natural polyphenolic compound, has various biological activities, such as antioxidative, anti-inflammatory, neuroprotective, hepatoprotective, nitric oxide radical-scavenging and vasodilative actions.
in vitro	Echinacoside behaves in vitro as an anti-oxidant and a potent free radical scavenger. It has been shown to rescue SH-SY5Y neuronal cells from TNF-alpha-induced apoptosis. This compound significantly attenuates glutamate-induced neurotoxicity and exhibits neuroprotective activities in vitro^[1]. It has significant neuroprotective effects on TNFα-induced apoptosis in SHSY5Y neuronal cells by maintaining mitochondrial function, decreasing the generation of reactive oxygen species, increasing the expression of the antiapoptotic protein Bcl-2 and inhibiting caspase-3 activity through an antioxidation mechanism^[2].
in vivo	Echinacoside has neuroprotective effects in the mouse MPTP model of Parkinson's disease. Treatment with this compound improves motor behavior and attenuated the depletion of striatal dopamine, DOPAC and HVA in MPTP-lesioned mice^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	SHSY5Y cells
	濃度	1, 10 or 100 μg/ml
	反応時間	36 h
	実験の流れ	Cell viability is determined by use of an MTT assay. Cells are seeded in 96-well plates at 1×10⁴ cells per well and grown to 70% confluence in culture medium. The medium is replaced by medium containing various concentrations of this compound (1, 10 or 100 μg/ml) and/or 100 ng/ml TNFα. A total of 5 mg/ml MTT is added to each well after 36 h, and the culture continued to incubate for another 4 h at 37 °C. After the medium has been removed, cells and dye crystals are solubilized with 200 μl dimethylsulfoxide (DMSO), and optical density is measured at 570 nm.
動物実験	動物モデル	Adult male C57BL/6 mice
	投薬量	5 and 20 mg/kg
	投与方法	p.o.

参考

https://pubmed.ncbi.nlm.nih.gov/17359968/
https://pubmed.ncbi.nlm.nih.gov/15556132/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Sirtuin1 Mediates the Protective Effects of Echinacoside against Sepsis-Induced Acute Lung Injury via Regulating the NOX4-Nrf2 Axis [ Antioxidants (Basel), 2023, 10.3390/antiox12111925]	PubMed: 38001778
Astragaloside IV and echinacoside benefit neuronal properties via direct effects and through upregulation of SOD1 astrocyte function in vitro [ Naunyn Schmiedebergs Arch Pharmacol, 2020, 10.1007/s00210-020-02022-w]	PubMed: 33219470

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。