Echinocystic acid

製品コードS3837 バッチS383701

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₃₀H₄₈O₄

分子量

472.70

CAS No.

510-30-5

Solubility (25°C)*

体外

DMSO

94 mg/mL (198.85 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	様々なハーブに豊富に含まれる天然トリテルペンであるEchinocystic acid（EA）は、抗炎症作用や抗酸化作用など、様々な薬理活性を示します。
in vitro	Echinocystic acid (EA) could induce apoptosis in human HepG2 cells, as characterized by DNA fragmentation, activation of caspase-3, -8, and -9, and PARP cleavage. EA induces the truncation of Bid protein and reduction of Bcl-2 protein. EA also causes the loss of mitochondrial membrane potential (DWm) and cytochrome c release from mitochondria to cytosol. Moreover, EA could activate c-Jun NH2-terminal kinase (JNK) and p38 kinase, and JNK-specific inhibitor SP600125 and p38 kinase-specific inhibitor SB200235 could block serial molecular events of EA-induced apoptosis such as Bid truncation, Bcl-2 reduction, cytochrome c release, caspase activation, and DNA fragmentation in HepG2 cells. EA inhibits HepG2 cell proliferation in a dose-dependent manner with an IC50 value of 45.4 μM at 24h treatment.
in vivo	Administration of Echinocystic acid (EA) is found to improve the maximum stress and Young’s modulus of femur in OVX rats. Micro-computed tomography analysis reveals that EA could improve the trabecular architecture, as shown by increasing the BV/TV, Tb.N, and Tb.Th in OVX rats. However, EA does not affect the body weight and uterine weight. EA has been shown to display an anti-inflammatory effect in different models of chronic inflammation in mice through the down-regulation of pro-inflammatory cytokines such as IL-1β, IL-18, TNF-α. EA attenuates reserpine-induced pain/depression dyad partially through regulating the biogenic amines levels and GluN2B receptors in the hippocampus.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HepG2 cells
	濃度	0, 15, 30, 45, 60, 100 μM
	反応時間	24h
	実験の流れ	The cell survival rate is measured by a MTT assay. HepG2 cells (2 ×10⁴/well) are incubated with different concentrations of EA in 96-well plates for 24h. MTT solution (5 mg/ml) is then added to each well and incubated for another 4h. The resulting MTT-formazan product is dissolved by the addition of 0.04N HCl-isopropanol solutions. Absorbance is measured at 595 nm with a microplate reader. The cell viability is expressed as the optical density ratio of the treatment to control.
動物実験	動物モデル	female ovariectomy (OVX) Sprague–Dawley rats
	投薬量	1, 5 or 15 mg/kg/day
	投与方法	ig

参考

https://pubmed.ncbi.nlm.nih.gov/15358141/
https://pubmed.ncbi.nlm.nih.gov/26317835/
https://pubmed.ncbi.nlm.nih.gov/26729203/

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。