Datasheet

生物学的記述

Specificity	EGR1 Antibody [N15L8] detects endogenous levels of total EGR1 protein.
Background	EGR1 (Early Growth Response 1) is a zinc finger transcription factor belonging to the EGR family of immediate-early genes, characterized by three Cys2-His2 zinc finger domains that enable specific binding to EGR response elements (EBS) in target gene promoters to regulate transcription. EGR1 features an N-terminal activation domain, a central repressive region, and C-terminal DNA-binding zinc finger motifs. Its promoter contains serum response elements (SREs) and CArG boxes, which facilitate rapid induction by stimuli such as growth factors via MAPK/ERK signaling. EGR1 acts as a key mediator linking extracellular signals to nuclear gene expression, driving processes such as cell proliferation, differentiation, apoptosis, and EMT through targets like cyclin D1, SNAIL, SLUG, PTEN, and p21, often via RAS-RAF-MEK-ERK-ELK1 cascades activated by ER stress or growth factors. EGR1 exhibits dual roles in cancer, acting as both an oncogene that promotes tumor proliferation, invasion, metastasis, and angiogenesis (e.g., via VEGFA) and as a suppressor that induces apoptosis. It is also dysregulated in cardiovascular conditions through inflammation and remodeling.

Specificity

EGR1 Antibody [N15L8] detects endogenous levels of total EGR1 protein.

Background

EGR1 (Early Growth Response 1) is a zinc finger transcription factor belonging to the EGR family of immediate-early genes, characterized by three Cys2-His2 zinc finger domains that enable specific binding to EGR response elements (EBS) in target gene promoters to regulate transcription. EGR1 features an N-terminal activation domain, a central repressive region, and C-terminal DNA-binding zinc finger motifs. Its promoter contains serum response elements (SREs) and CArG boxes, which facilitate rapid induction by stimuli such as growth factors via MAPK/ERK signaling. EGR1 acts as a key mediator linking extracellular signals to nuclear gene expression, driving processes such as cell proliferation, differentiation, apoptosis, and EMT through targets like cyclin D1, SNAIL, SLUG, PTEN, and p21, often via RAS-RAF-MEK-ERK-ELK1 cascades activated by ER stress or growth factors. EGR1 exhibits dual roles in cancer, acting as both an oncogene that promotes tumor proliferation, invasion, metastasis, and angiogenesis (e.g., via VEGFA) and as a suppressor that induces apoptosis. It is also dysregulated in cardiovascular conditions through inflammation and remodeling.

使用情報

Application

WB, IP, IF, ChIP

Dilution

WB	IP	IF	CHIP
1:1000	1:50	1:1600	1:50

Reactivity

Mouse, Rat, Human

Source

Rabbit Monoclonal Antibody

MW

75 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/30290239/
https://pubmed.ncbi.nlm.nih.gov/33842351/

Application Data

WB

Validated by Selleck

Lane 1: JHH-1, Lane 2: REC-1, Lane 3: Mouse brain, Lane 4: Rat brain