Ellagic Acid hydrate

製品コードS5516 バッチS551601

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₁₄H₆O₈.H₂O
	分子量	320.21	CAS No.	314041-08-2
Solubility (25°C)*	体外	DMSO	2 mg/mL (6.24 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Ellagic acid is a potent inhibitor of protein kinase CK2 with IC50s of 0.04, 2.9 and 3.5 μM for CK2, Lyn and PKA respectively. It shows potent antioxidant, anti-mutagenic and antidepressant properties.
in vitro	Ellagic acid (EA) is able to inhibit the growth of several cancer cells. EA inhibited cell proliferation in a dose- and time-dependent manner by arresting both cell lines at the G1 phase of the cell cycle, which were from elevating p53 and Cip1/p21 and decreasing cyclin D1 and E levels. EA also induced caspase-3-mediated apoptosis by increasing the Bax : Bcl-2 ratio and restored anoikis in ES-2 and PA-1 cells. It is well known to have a free radical scavenging activity^[1]. EA reversed epithelial to mesenchymal transition by up-regulating E-cadherin and down-regulating Vimentin^[2].
in vivo	A 90-day subchronic toxicity study further demonstrated that orally feeding EA (9.4, 19.1, 39.1 g/kg b.w., resp.) could not induce mortality or treatment-related clinical signs throughout the experimental period on F344 rats, indicating the low toxicity of EA to mammalians. Furthermore, EA exhibits potent anticancer and anticarcinogenesis activities towards breast, colorectal, oral, prostate, pancreatic, bladder, neuroblastoma, melanoma, and lymphoma cells^[1]. Treatment of PANC-1 xenografted mice with EA resulted in significant inhibition in tumor growth and prolong mice survival rate^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Ovarian carcinoma ES-2 and PA-1 cells
	濃度	10~100 μM
	反応時間	12, 24, 48 h
	実験の流れ	Ovarian carcinoma cell lines were plated at 100,000 cells in six-well tissue culture dishes. After 18 h of culture, cells were treated with different concentrations of DMSO-dissolved EA (0, 10, 25, 50, 75, or 100 μM) or chemotherapeutic drugs (doxorubicin, paraplatin, and paclitaxel) or a combination of both drugs. At the various time points, cells were collected by trypsinization and stained with trypan blue, and the cell number in suspension was counted in duplicate using a hemocytometer.
動物実験	動物モデル	PANC-1 xenografts (Balb C nude mice, 4-6 weeks old)
	投薬量	0, 10 mg/kg, 20 mg/kg and 40 mg/kg
	投与方法	oral gavage

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Non-catalytic allostery in α-TAT1 by a phospho-switch drives dynamic microtubule acetylation [ J Cell Biol, 2022, 221-11e202202100]	PubMed: 36222836

Non-catalytic allostery in α-TAT1 by a phospho-switch drives dynamic microtubule acetylation [ J Cell Biol, 2022, 221-11e202202100]

PubMed: 36222836

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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