Datasheet

生物学的記述

Specificity	EPAC2 Antibody [G13N1] detects endogenous levels of total EPAC2 protein.
Background	EPAC2 (Exchange Protein Directly Activated by cAMP 2, also known as RAPGEF4) is a multi-domain guanine nucleotide exchange factor (GEF) that senses cAMP independently of PKA to activate the small GTPases Rap1 and Rap2. EPAC2 features an N-terminal regulatory region containing two cyclic nucleotide-binding domains (cNBD-A and cNBD-B) and a DEP domain, as well as a C-terminal catalytic region with a CDC25 homology domain (CDC25-HD) and a REM domain. In its inactive state, the regulatory region masks the catalytic lobe, but cAMP binding induces conformational changes, particularly hinge bending near Phe435, that relieve autoinhibition and expose the Rap-binding site. The CDC25-HD then facilitates GDP/GTP exchange on Rap GTPases, activating pathways that control actin cytoskeleton dynamics, cell adhesion, and junction formation. EPAC2 is highly expressed in the brain, pancreas, neuroendocrine tissues, and heart, where it regulates insulin granule exocytosis via Rim2 complexes, synaptic plasticity, neurotransmitter release, and neuronal signaling. Dysregulation of EPAC2 is linked to type 2 diabetes (due to impaired insulin secretion), glioma development, and cardiovascular disorders.

Specificity

EPAC2 Antibody [G13N1] detects endogenous levels of total EPAC2 protein.

Background

EPAC2 (Exchange Protein Directly Activated by cAMP 2, also known as RAPGEF4) is a multi-domain guanine nucleotide exchange factor (GEF) that senses cAMP independently of PKA to activate the small GTPases Rap1 and Rap2. EPAC2 features an N-terminal regulatory region containing two cyclic nucleotide-binding domains (cNBD-A and cNBD-B) and a DEP domain, as well as a C-terminal catalytic region with a CDC25 homology domain (CDC25-HD) and a REM domain. In its inactive state, the regulatory region masks the catalytic lobe, but cAMP binding induces conformational changes, particularly hinge bending near Phe435, that relieve autoinhibition and expose the Rap-binding site. The CDC25-HD then facilitates GDP/GTP exchange on Rap GTPases, activating pathways that control actin cytoskeleton dynamics, cell adhesion, and junction formation. EPAC2 is highly expressed in the brain, pancreas, neuroendocrine tissues, and heart, where it regulates insulin granule exocytosis via Rim2 complexes, synaptic plasticity, neurotransmitter release, and neuronal signaling. Dysregulation of EPAC2 is linked to type 2 diabetes (due to impaired insulin secretion), glioma development, and cardiovascular disorders.

使用情報

Application

WB

Dilution

WB

1:1000

Reactivity

Human, Mouse, Rat

Source

Mouse Monoclonal Antibody

MW

115 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/25603503/
https://pubmed.ncbi.nlm.nih.gov/26390815/

Application Data

WB

Validated by Selleck

Lane 1: Mouse brain, Lane 2: Rat brain