受注:045-509-1970 |
技術サポート:[email protected] 平日9:00〜18:00 1営業日以内にご連絡を差し上げます |
Synonyms | (−)-ECG, (−)-Epicatechin 3-gallate, (−)-Epicatechin-3-O-gallate | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
|
化学式 | C22H18O10 |
|||
分子量 | 442.37 | CAS No. | 1257-08-5 | |
Solubility (25°C)* | 体外 | DMSO | 88 mg/mL (198.92 mM) | |
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | (-)-Epicatechin gallate ECG) is an inhibitor of cyclooxygenase-1 (COX-1) with IC50 of 7.5 μM. |
---|---|
in vitro | Treatment of HuH7 cells with (–)-Epigallocatechin gallate (EGCG) causes a time- and dose-dependent decrease in the expression of VEGFR-2 and p-VEGFR-2 proteins. EGCG effectively suppresses the growth of HuH7 human HCC cells both in vitro and in vivo[1]. EGCG potently modulates cardiac excitation contraction coupling by acting on RyR and possibly on L-type Ca2+-channel and NaCa exchanger[2]. |
in vivo | Drinking of EGCG significantly inhibits the growth of HuH7 xenografts in nude mice and this is associated with inhibition of the activation of VEGFR-2 and its related downstream signaling molecules, including ERK and Akt. EGCG drinking also decreases the expression of Bcl-xL protein and VEGF mRNA in the xenografts[1]. |
細胞アッセイ | 細胞株 | HuH7 or Hc cells |
---|---|---|
濃度 | 0-100 μg/mL | |
反応時間 | 48 h | |
実験の流れ | Three thousand HuH7 or Hc cells are seeded into 96-well plates. Twenty-four hours later, the cells are treated with the indicated concentrations of EGCG (0–100 μg/mL) for 48 h in DF10 medium, and cell viability is examined. All assays are carried out in triplicate. | |
動物実験 | 動物モデル | BALB/c nude mice (5 weeks of age) |
投薬量 | 0.01 or 0.1% | |
投与方法 | oral administration |
|
Glutamine metabolism regulates FLIP expression and sensitivity to TRAIL in triple-negative breast cancer cells. [ Cell Death Dis, 2018, 9(2):205] | PubMed: 29434187 |
Epigallocatechin-3 gallate prevents pressure overload-induced heart failure by up-regulating SERCA2a via histone acetylation modification in mice. [ PLoS One, 2018, 13(10):e0205123] | PubMed: 30286210 |
EGCG: Potential application as a protective agent against grass carp reovirus in aquaculture. [ J Fish Dis, 2018, 10.1111/jfd.12819] | PubMed: 29806139 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。