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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C16H25NO4 |
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分子量 | 295.37 | CAS No. | 81147-92-4 | |
Solubility (25°C)* | 体外 | DMSO | 59 mg/mL (199.74 mM) | |
Water | 59 mg/mL (199.74 mM) | |||
Ethanol | 59 mg/mL (199.74 mM) | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Esmolol is a cardioselective beta1 receptor blocker with rapid onset, a very short duration of action, and no significant intrinsic sympathomimetic or membrane stabilising activity at therapeutic dosages. |
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in vitro | Esmolol acts as a pharmacochaperone: Long term exposure of cells to esmolol raised the surface levels of β1-adrenergic receptors[1]. |
細胞アッセイ | 細胞株 | HEK293 cells expressing human β1- and β2-adrenergic receptors |
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濃度 | 1 μM | |
反応時間 | 40 min | |
実験の流れ | Cells are seeded into 6-cm dishes in DMEM containing 10% FCS, allowed to adhere for 8 hours, and then rendered quiescent by serum starvation for 16 hours. Cells are subsequently stimulated by addition of medium containing or lacking 1 μM esmolol, 1 μM landiolol, or 1 μM isoproterenol (as a positive control). Cells are maintained at 37°C for up to 40 minutes, the incubation is terminated by rapid rinsing with ice-cold PBS. Thereafter, the dish is immediately immersed in liquid nitrogen. After rapid thawing, cells were disrupted by addition of 80 μl of lysis buffer, 1% Nonidet P-40, 0.1% SDS, 250 IU/ml aprotinin, and 40 μg/ml leupeptin. The cellular debris was removed by centrifugation at 10,000g for 10 minutes, and the total protein content was measured photometrically using bicinchoninic acid. |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。