Estradiol Benzoate

製品コードS4110 バッチS411001

印刷

化学情報

Synonyms

β-Estradiol 3-benzoate

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₅H₂₈O₃

分子量

376.49

CAS No.

50-50-0

Solubility (25°C)*

体外

DMSO

19 mg/mL (50.46 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.950mg/ml (2.52mM)	Taking the 1 mL working solution as an example, add 50 μL of 19 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.500mg/ml (1.33mM)	Taking the 1 mL working solution as an example, add 50 μL of 10 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Estradiol benzoate(β-Estradiol 3-benzoate) is an estradiol analog which binds to the human, murine and chicken ERα with IC50 of 22-28 nM.
in vitro	Estradiol benzoate is asynthetic ester, specifically the 3-benzoyl ester, of the natural estrogen, estradiol. Estrogens direct the development of the female genotype in embryogenesis and at puberty. Estradiol is the major estrogen secreted by the premenopausal ovary. Estradiol benzoate is an estradiol analog which contains a benzyl ester at the C-3 position and is often used in combination with a progestin to induce estrus in domestic livestock. This compound binds to the human and murine estrogen receptor α (ERα), and chicken ER with IC50 values in the range of 22-28 nM. This reflects a 6-10 fold reduction in binding affinity compared to estradiol. ^[1]

製品説明

Estradiol benzoate(β-Estradiol 3-benzoate) is an estradiol analog which binds to the human, murine and chicken ERα with IC50 of 22-28 nM.

in vitro

Estradiol benzoate is asynthetic ester, specifically the 3-benzoyl ester, of the natural estrogen, estradiol. Estrogens direct the development of the female genotype in embryogenesis and at puberty. Estradiol is the major estrogen secreted by the premenopausal ovary. Estradiol benzoate is an estradiol analog which contains a benzyl ester at the C-3 position and is often used in combination with a progestin to induce estrus in domestic livestock. This compound binds to the human and murine estrogen receptor α (ERα), and chicken ER with IC50 values in the range of 22-28 nM. This reflects a 6-10 fold reduction in binding affinity compared to estradiol. ^[1]

プロトコル(参考用のみ)

キナーゼアッセイ	Receptor binding assays
キナーゼアッセイ	GST-ERdef fusion proteins are diluted in TEGD buffer (10 mM Tris pH 7.6, 1.5 mM EDTA, 1 mM DTT and 10% (v/v) glycerol) containing 1 mg/mL bovine serum albumin (BSA), and incubated at 4°C for 2 h with 0.1-3.5 nM [³H]E2 in 1 ml glass tubes arranged in a 96-well format. Fusion protein preparations are diluted to ensure 10000 dpms of total binding (varied from 750-2000-fold). Binding assays are initiated by adding 240 μL of protein preparation to glass tubes containing 5 μL of DMSO and 5 μL [³H]E2, thus the concentration of solvent does not exceed 4%. Bound [³H]E2 is separated from free using a 96-well filter plate and vacuum pump harvester. After drying, the filter plates are sealed and 50 μL of MicroScint 20 scintillation cocktail is added to each well. Bound [³H]E2 is measured using a TopCount luminescense and scintillation counter.

参考

https://pubmed.ncbi.nlm.nih.gov/11162928/

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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