Estradiol Benzoate

製品コードS4110 バッチS411001

印刷

化学情報

Chemical Structure Synonyms β-Estradiol 3-benzoate Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C25H28O3
分子量 376.49 CAS No. 50-50-0
Solubility (25°C)* 体外 DMSO 19 mg/mL (50.46 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
0.95mg/ml Taking the 1 mL working solution as an example, add 50 μL of 19 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Estradiol benzoate(β-Estradiol 3-benzoate) is an estradiol analog which binds to the human, murine and chicken ERα with IC50 of 22-28 nM.
in vitro

Estradiol benzoate is asynthetic ester, specifically the 3-benzoyl ester, of the natural estrogen, estradiol. Estrogens direct the development of the female genotype in embryogenesis and at puberty. Estradiol is the major estrogen secreted by the premenopausal ovary. Estradiol benzoate is an estradiol analog which contains a benzyl ester at the C-3 position and is often used in combination with a progestin to induce estrus in domestic livestock. This compound binds to the human and murine estrogen receptor α (ERα), and chicken ER with IC50 values in the range of 22-28 nM. This reflects a 6-10 fold reduction in binding affinity compared to estradiol. [1]

プロトコル(参考用のみ)

キナーゼアッセイ Receptor binding assays
GST-ERdef fusion proteins are diluted in TEGD buffer (10 mM Tris pH 7.6, 1.5 mM EDTA, 1 mM DTT and 10% (v/v) glycerol) containing 1 mg/mL bovine serum albumin (BSA), and incubated at 4°C for 2 h with 0.1-3.5 nM [3H]E2 in 1 ml glass tubes arranged in a 96-well format. Fusion protein preparations are diluted to ensure 10000 dpms of total binding (varied from 750-2000-fold). Binding assays are initiated by adding 240 μL of protein preparation to glass tubes containing 5 μL of DMSO and 5 μL [3H]E2, thus the concentration of solvent does not exceed 4%. Bound [3H]E2 is separated from free using a 96-well filter plate and vacuum pump harvester. After drying, the filter plates are sealed and 50 μL of MicroScint 20 scintillation cocktail is added to each well. Bound [3H]E2 is measured using a TopCount luminescense and scintillation counter.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Hearing Vocalizations during First Social Experience with Pups Increase Bdnf Transcription in Mouse Auditory Cortex [ Neural Plast, 2023, 2023:5225952] PubMed: 36845359
Investigation of Antiparasitic Activity of Two Marine Natural Products, Estradiol Benzoate, and Octyl Gallate, on Toxoplasma gondii In Vitro [ Front Pharmacol, 2022, 13:841941] PubMed: 35370702
Estradiol regulates intestinal ABCG2 to promote urate excretion via the PI3K/Akt pathway [ Nutr Metab (Lond), 2021, 18(1):63] PubMed: 34144706

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。