Eupatilin

製品コードS3846 バッチS384601

印刷

化学情報

Chemical Structure Synonyms NSC 122413 Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C18H16O7
分子量 344.32 CAS No. 22368-21-4
Solubility (25°C)* 体外 DMSO 68 mg/mL (197.49 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Eupatilin (NSC 122413), a major flavonoid from Artemisia plants, possesses various beneficial biological effects including anti-inflammation, anti-tumor, anti-cancer, anti-allergy, and anti-oxidation activity.
in vitro Eupatilin has been reported to induce apoptosis in human gastric cancer AGS cells, also triggers differentiation of these cells. Treatment of AGS cells with eupatilin induces cell cycle arrest at the G1 phase with the concomitant induction of p21cip1, a cell cycle inhibitor. It also markedly induces trefoil factor1(TFF1). Eupatilin dramatically induces redistribution of tight junction proteins such as occludin and ZO-1, and F-actin at the junctional region between cells. It also induces phosphorylation of extracellular signal-regulated kinase 2 and p38 kinase. Eupatilin is known to inhibit the growth of MCF-10A-ras cells by inhibiting the expression of cell cycle regulators such as cyclinD1, cyclinB1, Cdk2, and Cdc2[1].
in vivo Treatment with eupatilin significantly decreases serum alanine aminotransferase and serum aspartate aminotransferase as well as liver histologic changes. Eupatilin also preventes hepatic glutathione depletion and increases malondialdehyde levels induced by Ischemia-reperfusion injury (IRI). It improves the acute hepatic IRI by reducing inflammation and apoptosis[2]. Oral administration of eupatilin (10 mg/kg) in a therapeutic paradigm significantly reduces brain infarction and improves neurological functions in tMCAO-challenged mice. Eupatilin administration reduces the number of Iba1-immunopositive cells across ischemic brain and induces their morphological changes from amoeboid into ramified in the ischemic core, which is accompanied with reduced microglial proliferation in ischemic brain. Eupatilin suppresses NF-κB signaling activities in ischemic brain by reducing IKKα/β phosphorylation, IκBα phosphorylation, and IκBα degradation[3].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Human gastric epithelial AGS cells
濃度 10-500 μM
反応時間 24, 48, 72 h
実験の流れ Cellular viability is determined by PI incorporation. Cells are treated with various reagents, washed with PBS twice, resuspended in PBS containing 20 μg/ml PI, and then immediately analyzed on a FACSCalibur. PI-positive cells are counted as dead cells and the remaining cells are designated as viable cells.
動物実験 動物モデル C57BL/6 mice
投薬量 10 mg/kg
投与方法 oral

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Eupatilin Ameliorates Hepatic Fibrosis and Hepatic Stellate Cell Activation by Suppressing β-catenin/PAI-1 Pathway [ Int J Mol Sci, 2023, 24(6)5933] PubMed: 36983006
Eupatilin attenuates the senescence of nucleus pulposus cells and mitigates intervertebral disc degeneration via inhibition of the MAPK/NF-κB signaling pathway [ Front Pharmacol, 2022, 13:940475] PubMed: 36408239
β-Caryophyllene Acts as a Ferroptosis Inhibitor to Ameliorate Experimental Colitis [ Int J Mol Sci, 2022, 23(24)16055] PubMed: 36555694
Eupatilin Treatment Inhibits Transforming Growth Factor Beta-Induced Endometrial Fibrosis in Vitro [ Clin Exp Reprod Med, 2020, 47(2):108-113] PubMed: 32460455

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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