Euphorbiasteroid

製品コードS3832 バッチS383201

印刷

化学情報

Synonyms

Euphorbia factor L1

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₃₂H₄₀O₈

分子量

552.66

CAS No.

28649-59-4

Solubility (25°C)*

体外

DMSO

21 mg/mL (37.99 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Euphorbiasteroid (Euphorbia factor L1), a component of Euphorbia lathyris L., inhibits adipogenesis of 3T3-L1 cells through activation of the AMPK pathway and induces HL-60 cells to apoptosis via promoting Bcl-2/Bax apoptotic signaling pathway in a dose-dependent manner.
in vitro	Euphorbiasteroid suppresses adipogenic differentiation of 3T3‐L1 cells, mainly at the early stage, and stimulates the AMPK signalling pathway. The anti-adipogenic effects of this compound could possibly be attributed to activation of the AMPK pathway, by decreasing the level of FAS and its up-regulators, including C/EBPs, PPAR-γ and SREBP-1c, without involving insulin signalling pathway^[1]. This chemical could be a transport substrate for P-gp that can effectively inhibit P-gp-mediated drug transport and reverse resistance to anticancer drugs in MES-SA/Dx5 cells^[2].

製品説明

Euphorbiasteroid (Euphorbia factor L1), a component of Euphorbia lathyris L., inhibits adipogenesis of 3T3-L1 cells through activation of the AMPK pathway and induces HL-60 cells to apoptosis via promoting Bcl-2/Bax apoptotic signaling pathway in a dose-dependent manner.

in vitro

Euphorbiasteroid suppresses adipogenic differentiation of 3T3‐L1 cells, mainly at the early stage, and stimulates the AMPK signalling pathway. The anti-adipogenic effects of this compound could possibly be attributed to activation of the AMPK pathway, by decreasing the level of FAS and its up-regulators, including C/EBPs, PPAR-γ and SREBP-1c, without involving insulin signalling pathway^[1]. This chemical could be a transport substrate for P-gp that can effectively inhibit P-gp-mediated drug transport and reverse resistance to anticancer drugs in MES-SA/Dx5 cells^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	The murine pre-adipocyte cell line 3T3‐L1
	濃度	0-50 μM
	反応時間	2 days
	実験の流れ	3T3‐L1 cells were treated with euphorbiasteroid at concentrations of 6.25, 12.5, 25 and 50 µM for 2 days in adipogenesis induction medium, 2 days in adipogenesis medium and 2 days in culture medium (CM) sequentially during differentiation. Intracellular triglycerides (TGs) were stained with Oil red O (ORO) solution.

参考

https://pubmed.ncbi.nlm.nih.gov/25914364/
https://onlinelibrary.wiley.com/doi/full/10.1002/ptr.3073

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。