ex229 (compound 991)

製品コードS8654 バッチS865401

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₂₄H₁₈ClN₃O₃

分子量

431.87

CAS No.

1219739-36-2

Solubility (25°C)*

体外

DMSO

12 mg/mL (27.78 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	8%DMSO 1 40%PEG300＋5%Tween80＋47%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	1.160mg/ml (2.69mM)	Taking the 1 mL working solution as an example, add 80 μL of 14.5 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 470 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	EX229 (compound 991) is a potent AMPK activator that is 5-10-fold more potent than A769662 in activating AMPK.
in vitro	EX229 (compound 991) activates AMPK in incubated rat epitrochlearis skeletal muscle and incubation of rat skeletal muscle with it increases glucose uptake. The stimulation of skeletal muscle glucose uptake by this compound is PI3K/PKB-independent, while AMPK-dependent. It increases fatty acid oxidation and glucose uptake in L6 myotubes^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HEK293T cells
	濃度	50 μM
	反応時間	1 h
	実験の流れ	Cells transfected with Myc-Rabep1 WT or Ser407Ala were treated for 60 min with vehicle or 50 μM ex229 (compound 991).

参考

https://pubmed.ncbi.nlm.nih.gov/24665903/
https://pubmed.ncbi.nlm.nih.gov/31801093/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Alpha-synuclein mutations mislocalize cytoplasmic p300 compromising autophagy, which is rescued by ACLY inhibition [ Neuron, 2025, S0896-6273(25)00247-8]	PubMed: 40262613
Unexpected roles for AMPK in the suppression of autophagy and the reactivation of MTORC1 signaling during prolonged amino acid deprivation [ Autophagy, 2024, 1-24.]	PubMed: 38744665
AMPK agonism optimizes the in vivo persistence and anti-leukemia efficacy of chimeric antigen receptor T cells [ bioRxiv, 2024, 2024.09.26.615290]	PubMed: 39386600
CRISPR screens decode cancer cell pathways that trigger γδ T cell detection [ Nature, 2023, 621(7977):188-195]	PubMed: 37648854
Redefining the role of AMPK in autophagy and the energy stress response [ Nat Commun, 2023, 14(1):2994]	PubMed: 37225695
Activity-dependent subcellular compartmentalization of dendritic mitochondria structure in CA1 pyramidal neurons [ bioRxiv, 2023, 2023.03.25.534233]	PubMed: 36993655
[Fuyu Decoction improves ventricular remodeling in rats with heart failure by inhibiting AMPK/mTOR pathway-mediated autophagy] [ Nan Fang Yi Ke Da Xue Xue Bao, 2023, 43(3):466-473]	PubMed: 37087593
[Fuyu Decoction improves ventricular remodeling in rats with heart failure by inhibiting AMPK/mTOR pathway-mediated autophagy] [ Nan Fang Yi Ke Da Xue Xue Bao, 2023, 43(3):466-473]	PubMed: 37087593
Energy sensor AMPK gamma regulates translation via phosphatase PPP6C independent of AMPK alpha [ Mol Cell, 2022, S1097-2765(22)01058-9]	PubMed: 36384136
IL11 stimulates ERK/P90RSK to inhibit LKB1/AMPK and activate mTOR initiating a mesenchymal program in stromal, epithelial, and cancer cells [ iScience, 2022, 25(8):104806]	PubMed: 35992082

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。