ex229 (compound 991)

製品コードS8654 バッチS865401

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₂₄H₁₈ClN₃O₃
	分子量	431.87	CAS No.	1219739-36-2
Solubility (25°C)*	体外	DMSO	12 mg/mL (27.78 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	EX229 (compound 991) is a potent AMPK activator that is 5-10-fold more potent than A769662 in activating AMPK.
in vitro	EX229 activates AMPK in incubated rat epitrochlearis skeletal muscle and incubation of rat skeletal muscle with ex229 increases glucose uptake. The stimulation of skeletal muscle glucose uptake by ex229 is PI3K/PKB-independent, while AMPK-dependent. ex229 increases fatty acid oxidation and glucose uptake in L6 myotubes^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HEK293T cells
	濃度	50 μM
	反応時間	1 h
	実験の流れ	Cells transfected with Myc-Rabep1 WT or Ser407Ala were treated for 60 min with vehicle or 50 μM 991.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Redefining the role of AMPK in autophagy and the energy stress response [ Nat Commun, 2023, 14(1):2994]	PubMed: 37225695
Activity-dependent subcellular compartmentalization of dendritic mitochondria structure in CA1 pyramidal neurons [ bioRxiv, 2023, 2023.03.25.534233]	PubMed: 36993655
[Fuyu Decoction improves ventricular remodeling in rats with heart failure by inhibiting AMPK/mTOR pathway-mediated autophagy] [ Nan Fang Yi Ke Da Xue Xue Bao, 2023, 43(3):466-473]	PubMed: 37087593
Energy sensor AMPK gamma regulates translation via phosphatase PPP6C independent of AMPK alpha [ Mol Cell, 2022, S1097-2765(22)01058-9]	PubMed: 36384136
IL11 stimulates ERK/P90RSK to inhibit LKB1/AMPK and activate mTOR initiating a mesenchymal program in stromal, epithelial, and cancer cells [ iScience, 2022, 25(8):104806]	PubMed: 35992082
Liver kinase B1 (LKB1) in murine erythroid progenitors modulates erythropoietin setpoint in association with maturation control [ Blood Cells Mol Dis, 2022, 97:102688]	PubMed: 35717902
Quantitative In Vivo Proteomics of Metformin Response in Liver Reveals AMPK-Dependent and -Independent Signaling Networks. [ Cell Rep, 2019, 29(10):3331-3348]	PubMed: 31801093
To ameriorate the post-infarction heart failure in rats with a traditional Chinese medicine, Wenyang-Yiqi recipe, and to analyze its pharmacological mechanism by AMPK-mediated mitochondrial autophagy [ CHINESE JOURNAL OF COMPARATIVE MEDICINE, 2019, 29(12): 39-44]	PubMed: None

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。