FCCP

製品コードS8276 バッチS827603

印刷

化学情報

Synonyms

Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₀H₅F₃N₄O

分子量

254.17

CAS No.

370-86-5

Solubility (25°C)*

体外

DMSO

6 mg/mL (23.6 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone) is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes.
in vitro	FCCP treatment induces a very rapid 2-fold increase in intracellular Ca2+ concentration that is accompanied by a strong protein synthesis rate inhibition. The translation inhibition correlates with an increased phosphorylation of the α subunit of eIF2 (eIF2α) and a 1.7-fold increase in the double-stranded RNA-dependent protein kinase activity^[1]. This compound also mildly decreases ATP and reactive oxygen species levels. It increases the expression of mitochondrial genes such as Tfam and COXIV while inducing morphological features of quiescent mouse HSCs and abrogating TGF-β signal transduction^[2].
in vivo	FCCP significantly reduces mitochondrial membrane potential and ATP production in 8-cell mouse embryos and the number of inner cell mass cells within blastocysts with unchanged blastocyst development. This perturbed embryonic mitochondrial function is concomitant with reduced birth weight in female offspring following embryo transfer, which persists until weaning. Although this compound-treated males also exhibits reduced glucose tolerance as female, but their insulin sensitivity and adiposity gain between 4 and 14 weeks is unchanged. Reducing mitochondrial function and, thus, decreasing ATP output in the precompacting embryo can influence offspring phenotype^[3].

製品説明

FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone) is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes.

in vitro

FCCP treatment induces a very rapid 2-fold increase in intracellular Ca2+ concentration that is accompanied by a strong protein synthesis rate inhibition. The translation inhibition correlates with an increased phosphorylation of the α subunit of eIF2 (eIF2α) and a 1.7-fold increase in the double-stranded RNA-dependent protein kinase activity^[1].

This compound also mildly decreases ATP and reactive oxygen species levels. It increases the expression of mitochondrial genes such as Tfam and COXIV while inducing morphological features of quiescent mouse HSCs and abrogating TGF-β signal transduction^[2].

in vivo

FCCP significantly reduces mitochondrial membrane potential and ATP production in 8-cell mouse embryos and the number of inner cell mass cells within blastocysts with unchanged blastocyst development. This perturbed embryonic mitochondrial function is concomitant with reduced birth weight in female offspring following embryo transfer, which persists until weaning. Although this compound-treated males also exhibits reduced glucose tolerance as female, but their insulin sensitivity and adiposity gain between 4 and 14 weeks is unchanged. Reducing mitochondrial function and, thus, decreasing ATP output in the precompacting embryo can influence offspring phenotype^[3].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	PC12 cells
	濃度	30 μM
	反応時間	30 min, 1h, 2h
	実験の流れ	Protein synthesis rate is assayed in 24-mm diameter multi-well dishes with fresh medium containing 0.175 Ci/mmol of [3H]methionine (200 μM), for 30 min at 37°C. PC12 cells are treated with this compound for different period of times.
動物実験	動物モデル	Female C57BL/6 mice
	投薬量	1 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/11248255/
https://pubmed.ncbi.nlm.nih.gov/22686625/
https://pubmed.ncbi.nlm.nih.gov/25715796/

https://pubmed.ncbi.nlm.nih.gov/35163244/

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カスタマーフィードバック

: Data from [Data independently produced by , , J Pineal Res, 2018, 64:e12450]

: Data from [Data independently produced by , , Redox Biology, 2018, 14:576-587]

: Data from [Data independently produced by , , Redox Biology, 2018, 14:59-71]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Immune evasion through mitochondrial transfer in the tumour microenvironment [ Nature, 2025, 10.1038/s41586-024-08395-9]	PubMed: 39843734
Medium from human iPSC-derived primitive macrophages promotes adult cardiomyocyte proliferation and cardiac regeneration [ Nat Commun, 2025, 16(1):3012]	PubMed: 40148355
Menin maintains lysosomal and mitochondrial homeostasis through epigenetic mechanisms in lung cancer [ Cell Death Dis, 2025, 16(1):163]	PubMed: 40057469
OASL enhances mRNA translation and reprograms lipid metabolism to promote cancer progression [ Cell Rep, 2025, 44(7):115901]	PubMed: 40608514
MANF facilitates breast cancer cell survival under glucose-starvation conditions via PRKN-mediated mitophagy regulation [ Autophagy, 2024, 1-22.]	PubMed: 39147386
The DNA-dependent protein kinase catalytic subunit exacerbates endotoxemia-induced myocardial microvascular injury by disrupting the MOTS-c/JNK pathway and inducing profilin-mediated lamellipodia degradation [ Theranostics, 2024, 14(4):1561-1582]	PubMed: 38389837
Knockdown of hepatic mitochondrial calcium uniporter mitigates MASH and fibrosis in mice [ Cell Biosci, 2024, 14(1):135]	PubMed: 39523398
Pseudouridine synthase 1 regulates erythropoiesis via transfer RNAs pseudouridylation and cytoplasmic translation [ iScience, 2024, 27(3):109265]	PubMed: 38450158
A pharmacological toolkit for human microglia identifies Topoisomerase I inhibitors as immunomodulators for Alzheimer's disease [ bioRxiv, 2024, 2024.02.06.579103]	PubMed: 38370689
HIGD1A links SIRT1 activity to adipose browning by inhibiting the ROS/DNA damage pathway [ Cell Rep, 2023, 42(7):112731]	PubMed: 37393616

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。