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Synonyms | Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C10H5F3N4O |
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分子量 | 254.17 | CAS No. | 370-86-5 | ||||||||
Solubility (25°C)* | 体外 | DMSO | 51 mg/mL (200.65 mM) | ||||||||
Ethanol | 51 mg/mL (200.65 mM) | ||||||||||
Water | Insoluble | ||||||||||
体内 (毎回新しく調製した物を用意してください) |
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone) is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes. |
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in vitro | FCCP treatment induces a very rapid 2-fold increase in intracellular Ca2+ concentration that is accompanied by a strong protein synthesis rate inhibition. The translation inhibition correlates with an increased phosphorylation of the α subunit of eIF2 (eIF2α) and a 1.7-fold increase in the double-stranded RNA-dependent protein kinase activity[1]. FCCP treatment also mildly decreases ATP and reactive oxygen species levels. It increases the expression of mitochondrial genes such as Tfam and COXIV while inducing morphological features of quiescent mouse HSCs and abrogating TGF-β signal transduction[2]. |
in vivo | FCCP significantly reduces mitochondrial membrane potential and ATP production in 8-cell mouse embryos and the number of inner cell mass cells within blastocysts with unchanged blastocyst development. This perturbed embryonic mitochondrial function is concomitant with reduced birth weight in female offspring following embryo transfer, which persists until weaning. Although FCCP-treated males also exhibits reduced glucose tolerance as female, but their insulin sensitivity and adiposity gain between 4 and 14 weeks is unchanged. Reducing mitochondrial function and, thus, decreasing ATP output in the precompacting embryo can influence offspring phenotype[3]. |
細胞アッセイ | 細胞株 | PC12 cells |
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濃度 | 30 μM | |
反応時間 | 30 min, 1h, 2h | |
実験の流れ | Protein synthesis rate is assayed in 24-mm diameter multi-well dishes with fresh medium containing 0.175 Ci/mmol of [3H]methionine (200 μM), for 30 min at 37°C. PC12 cells are treated with FCCP for different period of times. |
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動物実験 | 動物モデル | Female C57BL/6 mice |
投薬量 | 1 mg/kg | |
投与方法 | i.p. |
Data from [Data independently produced by , , J Pineal Res, 2018, 64:e12450]
Data from [Data independently produced by , , Redox Biology, 2018, 14:576-587]
Data from [Data independently produced by , , Redox Biology, 2018, 14:59-71]
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長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。