Fer-1 (Ferrostatin-1)

製品コードS7243 バッチS724302

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₅H₂₂N₂O₂

分子量

262.35

CAS No.

347174-05-4

Solubility (25°C)*

体外

DMSO

55 mg/mL (209.64 mM)

Ethanol

55 mg/mL (209.64 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution

5% DMSO 1 40% PEG 300 2 5%tween 80 3 50%ddH2O

1.0mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O Dilute to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Fer-1 (Ferrostatin-1) is a potent and selective inhibitor of ferroptosis with EC50 of 60 nM.
in vitro	Ferrostatin-1 (2 μM) prevents erastin-induced ferroptosis in cancer cells, as well as glutamate-induced cell death in postnatal rat brain slices. Ferrostatin-1 is a lipid ROS scavenger, with the N-cyclohexyl moiety serving as a lipo-philic anchor within biological membranes. Ferrostatin-1 does not inhibit extracellular signal -regulated kinase (ERK) phos-phorylation or arrest the proliferation of HT-1080 cells, suggesting that it does not inhibit the MEK/ERK pathway, chelate iron, or inhibit protein synthesis. Ferrostatin-1 does, however, prevent erastin-induced accumulation of cytosolic and lipid ROS. Ferrostatin-1 readily oxidizes the stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) under cell-free conditions. ^[1]
in vivo	Ferrostatin-1 (Fer-1), a synthetic antioxidant, is a potent and selective inhibitor of ferroptosis. It acts via a reductive mechanism to prevent damage to membrane lipids and thereby inhibits cell death.^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HT-1080 cells
	濃度	60 nM (EC50)
	反応時間	24 h
	実験の流れ	Erastin treated cells were incubated with ferrostatin-1 at 37˚C for 24 hr and viability was assessed using Alamar Blue assay.
動物実験	動物モデル	C57BL/6 mice
	投薬量	10 mg/kg
	投与方法	i.n.

参考

カスタマーフィードバック

: Data from [Data independently produced by , , Hepatology, 2016, 64(2):488-500]

: Data from [Data independently produced by , , Cancer Res, 2017, 77(8):2064-2077]

: Data from [Data independently produced by , , Oncogene, 2015, 10.1038/onc.2015.32]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

7-Dehydrocholesterol dictates ferroptosis sensitivity [ Nature, 2024, 626(7998):411-418.]	PubMed: 38297130
Targeting of vulnerabilities of drug-tolerant persisters identified through functional genetics delays tumor relapse [ Cell Rep Med, 2024, 5(3):101471]	PubMed: 38508142
Angiotension II directly bind P2X7 receptor to induce myocardial ferroptosis and remodeling by activating human antigen R [ Redox Biol, 2024, 72:103154]	PubMed: 38626575
Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis [ Redox Biol, 2024, 69:103002]	PubMed: 38142583
FTO ameliorates doxorubicin-induced cardiotoxicity by inhibiting ferroptosis via P53-P21/Nrf2 activation in a HuR-dependent m6A manner [ Redox Biol, 2024, 70:103067]	PubMed: 38316068
Meningioma achieves malignancy and erastin-induced ferroptosis resistance through FOXM1-AURKA-NRF2 axis [ Redox Biol, 2024, 72:103137]	PubMed: 38642502
Protein phosphatase 1 regulatory subunit 15 A promotes translation initiation and induces G2M phase arrest during cuproptosis in cancers [ Cell Death Dis, 2024, 15(2):149]	PubMed: 38365764
Salidroside pretreatment alleviates ferroptosis induced by myocardial ischemia/reperfusion through mitochondrial superoxide-dependent AMPKα2 activation [ Phytomedicine, 2024, 155365]	PubMed: none
Vitamin B6 alleviates chronic sleep deprivation-induced hippocampal ferroptosis through CBS/GSH/GPX4 pathway [ Biomed Pharmacother, 2024, 174:116547]	PubMed: 38599059
Blocking CIRP protects against acute pancreatitis by improving mitochondrial function and suppressing pyroptosis in acinar cells [ Cell Death Discov, 2024, 10(1):156]	PubMed: 38538578

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。