Ferrostatin-1 (Fer-1)

製品コードS7243 バッチS724303

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₅H₂₂N₂O₂

分子量

262.35

CAS No.

347174-05-4

Solubility (25°C)*

体外

DMSO

100 mg/mL (381.17 mM)

Ethanol

52 mg/mL (198.2 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	2% DMSO 1 50% PEG 300 2 5% Tween 80 3 ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	1.000mg/ml (3.81mM)	Taking the 1 mL working solution as an example, add 20 μL of 50 mg/ml clarified DMSO stock solution to 500 μL PEG300, mix evenly to clarify it; add 50 μL Tween-80 to the above system, mix evenly to clarify it; Then continue to add 430 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	5.000mg/ml (19.06mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	1.650mg/ml (6.29mM)	Taking the 1 mL working solution as an example, add 50 μL of 33 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Fer-1 (Ferrostatin-1) は、フェロトーシスの強力かつ選択的な阻害剤であり、EC50 は 60 nM です。
in vitro	Ferrostatin-1 (Fer-1) (2 μM) prevents erastin-induced ferroptosis in cancer cells, as well as glutamate-induced cell death in postnatal rat brain slices. It is a lipid ROS scavenger, with the N-cyclohexyl moiety serving as a lipo-philic anchor within biological membranes. This compound does not inhibit extracellular signal -regulated kinase (ERK) phos-phorylation or arrest the proliferation of HT-1080 cells, suggesting that it does not inhibit the MEK/ERK pathway, chelate iron, or inhibit protein synthesis. It does, however, prevent erastin-induced accumulation of cytosolic and lipid ROS. Ferrostatin-1 readily oxidizes the stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) under cell-free conditions.
in vivo	Ferrostatin-1 (Fer-1), a synthetic antioxidant, is a potent and selective inhibitor of ferroptosis. It acts via a reductive mechanism to prevent damage to membrane lipids and thereby inhibits cell death.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HT-1080 cells
	濃度	60 nM (EC50)
	反応時間	24 h
	実験の流れ	After treatment with erastin, cells were incubated with ferrostatin-1 (Fer-1) at 37˚C for 24 hr, and viability was assessed using Alamar Blue assay.
動物実験	動物モデル	C57BL/6 mice
	投薬量	10 mg/kg
	投与方法	i.n.

参考

https://pubmed.ncbi.nlm.nih.gov/22632970/
https://pubmed.ncbi.nlm.nih.gov/35197442/

カスタマーフィードバック

: Data from [Data independently produced by , , Hepatology, 2016, 64(2):488-500]

: Data from [Data independently produced by , , Cancer Res, 2017, 77(8):2064-2077]

: Data from [Data independently produced by , , Oncogene, 2015, 10.1038/onc.2015.32]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Ferroptosis-Mediated Hippocampal Neuronal Loss Post-mTBI: Chromatin Accessibility Profiling and Single-Nucleus Transcriptomics [ Adv Sci (Weinh), 2026, 13(12):e12362]	PubMed: 41397940
Homoisoflavanone Delays Colorectal Cancer Progression via DNA Damage-Induced Mitochondrial Apoptosis and Parthanatos-Like Cell Death [ Adv Sci (Weinh), 2026, 13(19):e11406]	PubMed: 41603109
FAAH initiates a positive feedback loop to promote lung adenocarcinoma progression through inhibition of ferroptosis [ Cell Death Differ, 2026, 10.1038/s41418-026-01742-5 10.1038/s41571-023-00798-3 10.1158/0008-5472.CAN-24-1607 10.1126/scitranslmed.abk2756 10.1038/s41580-024-00703-5 10.1038/s4]	PubMed: 41998116
H3K18 lactylation-mediated SPHK1-SIRT1 feedback loop accelerates pyroptosis of tubular epithelial cells in sepsis-associated acute kidney injury [ Theranostics, 2026, 16(9):4768-4786]	PubMed: 41799201
Slc7a11-Mediated Cystine/Glutamate Antiport Reprograms Macrophage Polarization and Ameliorates Atherosclerosis [ MedComm (2020), 2026, 7(3):e70646]	PubMed: 41777250
Ipriflavone From Aquilaria malaccensis Lam. Exosome-Like Nanoparticles Targets Prolyl Hydroxylase Domain Protein 2 (PHD2) to Enhance Hypoxia-Inducible Factor-α (HIF-α) Hydroxylation Thereby Alleviating Hypoxia-Induced Gastrointestinal Mucosal Ferroptosis [ MedComm (2020), 2026, 7(4):e70722]	PubMed: 41969632
Ferroptosis modulates invasion and migration in prostate cancer PC-3M subclones [ Exp Cell Res, 2026, 454(2):114847]	PubMed: 41325788
Epstein-Barr Virus Latent Membrane Protein 1 Suppresses Ferroptosis via Pentose Phosphate Pathway and Glutathione Metabolism [ bioRxiv, 2026, 2026.03.09.710628]	PubMed: 41959453
Inhibition of heme biosynthesis triggers cuproptosis in acute myeloid leukemia [ Cell, 2025, S0092-8674(25)01233-4]	PubMed: 41265435
Lymphoma accelerates T cell and tissue aging [ Cancer Cell, 2025, S1535-6108(25)00329-0]	PubMed: 40845845

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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