Ferrostatin-1 (Fer-1)

製品コードS7243 バッチS724304

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C15H22N2O2
分子量 262.35 CAS No. 347174-05-4
Solubility (25°C)* 体外 DMSO 52 mg/mL (198.2 mM)
Ethanol 52 mg/mL (198.2 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Fer-1 (Ferrostatin-1) is a potent and selective inhibitor of ferroptosis with EC50 of 60 nM.
in vitro

Ferrostatin-1 (Fer-1) (2 μM) prevents erastin-induced ferroptosis in cancer cells, as well as glutamate-induced cell death in postnatal rat brain slices. It is a lipid ROS scavenger, with the N-cyclohexyl moiety serving as a lipo-philic anchor within biological membranes. This compound does not inhibit extracellular signal -regulated kinase (ERK) phos-phorylation or arrest the proliferation of HT-1080 cells, suggesting that it does not inhibit the MEK/ERK pathway, chelate iron, or inhibit protein synthesis. It does, however, prevent erastin-induced accumulation of cytosolic and lipid ROS, and readily oxidizes the stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) under cell-free conditions. [1]
in vivo

A potent and selective inhibitor of ferroptosis, Ferrostatin-1 (Fer-1) is a synthetic antioxidant. It acts via a reductive mechanism to prevent damage to membrane lipids and thereby inhibits cell death.[2]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HT-1080 cells
濃度 60 nM (EC50)
反応時間 24 h
実験の流れ

Erastin treated cells were incubated with ferrostatin-1 (Fer-1) at 37˚C for 24 hr and viability was assessed using Alamar Blue assay.
動物実験 動物モデル C57BL/6 mice
投薬量 10 mg/kg
投与方法 i.n.

参考

  • https://pubmed.ncbi.nlm.nih.gov/22632970/
  • https://pubmed.ncbi.nlm.nih.gov/35197442/

カスタマーフィードバック

Data from [Data independently produced by , , Hepatology, 2016, 64(2):488-500]

Data from [Data independently produced by , , Cancer Res, 2017, 77(8):2064-2077]

Data from [Data independently produced by , , Oncogene, 2015, 10.1038/onc.2015.32]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Inhibition of heme biosynthesis triggers cuproptosis in acute myeloid leukemia [ Cell, 2025, S0092-8674(25)01233-4] PubMed: 41265435
Lymphoma accelerates T cell and tissue aging [ Cancer Cell, 2025, S1535-6108(25)00329-0] PubMed: 40845845
Radiotherapy promotes cuproptosis and synergizes with cuproptosis inducers to overcome tumor radioresistance [ Cancer Cell, 2025, S1535-6108(25)00132-1] PubMed: 40215978
Cytosolic cytochrome c represses ferroptosis [ Cell Metab, 2025, S1550-4131(25)00149-4] PubMed: 40233758
RIPK1 senses S-adenosylmethionine scarcity to drive cell death and inflammation [ Cell Metab, 2025, S1550-4131(25)00294-3] PubMed: 40570842
Harnessing the FGFR2/NF2/YAP signaling-dependent necroptosis to develop an FGFR2/IL-8 dual blockade therapeutic strategy [ Nat Commun, 2025, 16(1):4128] PubMed: 40319089
Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis [ Nat Commun, 2025, 16(1):6617] PubMed: 40731111
Obesity-associated macrophages dictate adipose stem cell ferroptosis and visceral fat dysfunction by propagating mitochondrial fragmentation [ Nat Commun, 2025, 16(1):7564] PubMed: 40813577
S100P is a ferroptosis suppressor to facilitate hepatocellular carcinoma development by rewiring lipid metabolism [ Nat Commun, 2025, 16(1):509] PubMed: 39779666
Iron supplementation alleviates pathologies in a mouse model of facioscapulohumeral muscular dystrophy [ J Clin Invest, 2025, e181881] PubMed: 40591405

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。